X-ray-enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex.
X-ray-enhanced cancer cell migration requires the linker of nucleoskeleton and cytoskeleton complex.
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DOI：
10.1111/cas.13545
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发表时间：
2018-04
期刊：
影响因子：
5.7
通讯作者：
Hieda M
中科院分区：
文献类型：
--
作者：
Imaizumi H;Sato K;Nishihara A;Minami K;Koizumi M;Matsuura N;Hieda M
核骨架与细胞骨架连接体（linker of nucleoskeleton and cytoskeleton，LINC）复合物是一种多功能的蛋白复合物，参与核膜上的多种过程，包括核迁移、机械传递、染色质束缚和DNA损伤反应。我们最近发现，核膜蛋白，Sad 1和UNC 84结构域蛋白1（SUN 1），LINC复合物的一个组成部分，在细胞迁移中具有关键作用。尽管电离辐射在体内和体外激活细胞迁移和侵袭，但其潜在的分子机制仍不清楚。在这里，我们研究了LINC复合物在辐射增强的细胞迁移和侵袭中的参与。亚致死剂量的X射线辐射促进人乳腺癌MDA-MB-231细胞迁移和侵袭，而碳离子束辐射以剂量依赖性方式抑制这些过程。SUN 1和SUN 2的耗尽显著抑制了X射线增强的细胞迁移和侵袭。此外，每个SUN 1剪接变体的缺失或过表达揭示了含有SUN 1的888个氨基酸的SUN1_888而不是SUN1_916是X射线增强的迁移和侵袭所必需的。此外，结果表明，X射线照射影响了SUN 1剪接变体和SUN蛋白结合伴侣nesprins的表达水平。总之，我们的研究结果支持LINC复合物有助于光子增强的细胞迁移和侵袭。
The linker of nucleoskeleton and cytoskeleton (LINC) complex is a multifunctional protein complex that is involved in various processes at the nuclear envelope, including nuclear migration, mechanotransduction, chromatin tethering and DNA damage response. We recently showed that a nuclear envelope protein, Sad1 and UNC84 domain protein 1 (SUN1), a component of the LINC complex, has a critical function in cell migration. Although ionizing radiation activates cell migration and invasion in vivo and in vitro, the underlying molecular mechanism remains unknown. Here, we examined the involvement of the LINC complex in radiation‐enhanced cell migration and invasion. A sublethal dose of X‐ray radiation promoted human breast cancer MDA‐MB‐231 cell migration and invasion, whereas carbon ion beam radiation suppressed these processes in a dose‐dependent manner. Depletion of SUN1 and SUN2 significantly suppressed X‐ray‐enhanced cell migration and invasion. Moreover, depletion or overexpression of each SUN1 splicing variant revealed that SUN1_888 containing 888 amino acids of SUN1 but not SUN1_916 was required for X‐ray‐enhanced migration and invasion. In addition, the results suggested that X‐ray irradiation affected the expression level of SUN1 splicing variants and a SUN protein binding partner, nesprins. Taken together, our findings supported that the LINC complex contributed to photon‐enhanced cell migration and invasion.
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影响因子：
8.4
作者：
Jung, Jae-Won;Hwang, So-Young;Han, Inn-Oc
通讯作者：
Han, Inn-Oc
影响因子：
9.2
作者：
Lei, Kai;Zhu, Xiaoqiang;Han, Min
通讯作者：
Han, Min
DOI：
10.4161/19491034.2014.990857
发表时间：
2015
期刊：
Nucleus (Austin, Tex.)
影响因子：
--
作者：
Cain NE;Starr DA
通讯作者：
Starr DA
影响因子：
8
作者：
Cheng, J. C-H;Chou, C. H.;Hsieh, C-Y
通讯作者：
Hsieh, C-Y
影响因子：
7.8
作者：
Crisp, M;Liu, Q;Hodzic, D
通讯作者：
Hodzic, D