Spatial analysis of biomineralization associated gene expression from the mantle organ of the pearl oyster Pinctada maxima.
Spatial analysis of biomineralization associated gene expression from the mantle organ of the pearl oyster Pinctada maxima.
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DOI：
10.1186/1471-2164-12-455
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发表时间：
2011-09-21
期刊：
影响因子：
4.4
通讯作者：
Elizur A
中科院分区：
文献类型：
--
作者：
Gardner LD;Mills D;Wiegand A;Leavesley D;Elizur A
生物矿化是指生物体中产生的所有含矿物质组织的过程。这一过程中最具活力的例子之一是软体动物壳的形成，包括各种晶体相和微观结构。结合在外壳内的有机组件据说决定了这种体系结构。然而，对这一过程如何实现的一般理解仍然模糊不清。在整个软体动物的壳形成过程中，地幔是一个保守的器官。特别是地幔被认为是负责分泌壳的蛋白质成分。本研究采用分子方法确定了地幔组织内基因的空间表达，以进一步阐明壳生物矿化。以珍珠牡蛎（Pinctada maxima）为样本，定制微阵列平台（PmaxArray 1.0）。PmaxArray 1.0包含4992个源自地幔组织的est。该芯片用于分析est在整个套器官中的空间表达。利用PmaxArray 1.0对其进行基因差异表达分析。2000多个est在组织切片中存在差异表达，确定了5个主要表达区。原位杂交验证并进一步定位了这些ESTs的一部分表达。比较序列相似性分析显示，许多转录本是新的，而其他转录本则显示出与先前表征的壳相关基因的显著序列相似性。本研究在PmaxArray 1.0上绘制了2000多个est的空间分布，并参考了地幔的特定位置。表达剖面簇显示了地幔中至少五个独特的功能区。其中三个区域可能与贝壳相关的活动有关，包括珠层、骨膜和方解柱微观结构的形成。许多新的和已知的转录本已经从这些集群中确定。PmaxArray 1.0的开发及其在地幔中est表达的空间图谱已经开始表征软体动物壳中有机物和无机物的分子机制。
Biomineralization is a process encompassing all mineral containing tissues produced within an organism. One of the most dynamic examples of this process is the formation of the mollusk shell, comprising a variety of crystal phases and microstructures. The organic component incorporated within the shell is said to dictate this architecture. However general understanding of how this process is achieved remains ambiguous. The mantle is a conserved organ involved in shell formation throughout molluscs. Specifically the mantle is thought to be responsible for secreting the protein component of the shell. This study employs molecular approaches to determine the spatial expression of genes within the mantle tissue to further the elucidation of the shell biomineralization. A microarray platform was custom generated (PmaxArray 1.0) from the pearl oyster Pinctada maxima. PmaxArray 1.0 consists of 4992 expressed sequence tags (ESTs) originating from mantle tissue. This microarray was used to analyze the spatial expression of ESTs throughout the mantle organ. The mantle was dissected into five discrete regions and analyzed for differential gene expression with PmaxArray 1.0. Over 2000 ESTs were determined to be differentially expressed among the tissue sections, identifying five major expression regions. In situ hybridization validated and further localized the expression for a subset of these ESTs. Comparative sequence similarity analysis of these ESTs revealed a number of the transcripts were novel while others showed significant sequence similarities to previously characterized shell related genes. This investigation has mapped the spatial distribution for over 2000 ESTs present on PmaxArray 1.0 with reference to specific locations of the mantle. Expression profile clusters have indicated at least five unique functioning zones in the mantle. Three of these zones are likely involved in shell related activities including formation of nacre, periostracum and calcitic prismatic microstructure. A number of novel and known transcripts have been identified from these clusters. The development of PmaxArray 1.0, and the spatial map of its ESTs expression in the mantle has begun characterizing the molecular mechanisms linking the organics and inorganics of the molluscan shell.
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影响因子：
2.5
作者：
Kramerova, IA;Kramerov, AA;Fessler, JH
通讯作者：
Fessler, JH
影响因子：
4.4
作者：
Joubert C;Piquemal D;Marie B;Manchon L;Pierrat F;Zanella-Cléon I;Cochennec-Laureau N;Gueguen Y;Montagnani C
通讯作者：
Montagnani C
影响因子：
2.1
作者：
Kendziorski, CM;Zhang, Y;Attie, AD
通讯作者：
Attie, AD
影响因子：
1.9
作者：
Miyamoto, H;Hamaguchi, M;Okoshi, K
通讯作者：
Okoshi, K
DOI：
10.1006/bbrc.2000.2274
发表时间：
2000-03-05
影响因子：
3.1
作者：
Kono, M;Hayashi, N;Samata, T
通讯作者：
Samata, T