Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration.
Evaluation of stability and sensitivity of cell fluorescent labels when used for cell migration.
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DOI：
10.1007/s10895-013-1224-8
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发表时间：
2013-09
影响因子：
2.7
通讯作者：
Segal, Mark S.
中科院分区：
文献类型：
--
作者：
Beem, Elaine;Segal, Mark S.
关键词：
哺乳动物细胞的定向迁移是发育和生长的基础。多种过程，如组织发育、伤口愈合、病原体识别/破坏以及癌症转移都是细胞迁移调节或失调的结果。虽然测量细胞迁移倾向的能力在几种情况下具有临床相关性，但尚未建立测量细胞迁移的通用方案。目前有多种技术用于测量迁移，包括人工计数，流式细胞术或库尔特计数，微流体装置，计算机化光谱方法，或使用与荧光或非荧光板读取器接口的各种跟踪染料。为了加快迁移的测量，我们比较了几种常见的细胞质和亲脂性细胞跟踪染料，以确定用于测定罕见细胞迁移的最佳染料。CellVue®Burgundy在细胞内的保留优于calcein AM， Cell Tracker Green CMFDA（氯甲基荧光素二乙酸酯），Vybrant CFDA（羧基荧光素二乙酸琥珀酰酯），优于CellVue®NIR 815， PKH67和CM DiI的信噪比，优于PKH26的仪器多功能性。
The directed migration of mammalian cells is a foundation of development and growth. A variety of processes such as tissue development, wound healing, pathogen recognition/destruction as well as cancer metastasis are the result of regulated or dysregulated cell migration. While the ability to measure a cell's propensity to migrate has clinical relevance in several settings, no universal protocol has been established to measure cell migration. A variety of techniques are currently used to measure migration including manual counting, flow cytometry or Coulter counting, microfluidic devices, computerized spectroscopic methods, or the use of various tracking dyes interfaced with fluorescent or non-fluorescent plate readers. In order to expedite the measurement of migration, we compared several common cytoplasmic and lipophilic cell tracking dyes to determine the best dye for determining migration of rare population of cells. CellVue® Burgundy was found to be superior over calcein AM, Cell Tracker Green CMFDA (chloromethyl fluorescein diacetate), Vybrant CFDA (carboxy fluorescein diacetate succinimidyl ester) in its retention within cells, superior to CellVue® NIR 815, PKH67, and CM DiI with regard to signal to noise ratio, and superior to PKH26 with regard to instrument versatility.
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影响因子：
7.7
作者：
Segal, MS;Shah, R;Grant, MB
通讯作者：
Grant, MB
影响因子：
19.6
作者：
Hauser, IA;Koziolek, M;Thévenod, F
通讯作者：
Thévenod, F
DOI：
10.1073/pnas.84.21.7735
发表时间：
1987-11-01
影响因子：
11.1
作者：
THIEBAUT, F;TSURUO, T;WILLINGHAM, MC
通讯作者：
WILLINGHAM, MC
影响因子：
2.9
作者：
Essodaïgui, M;Broxterman, HJ;Garnier-Suillerot, A
通讯作者：
Garnier-Suillerot, A
DOI：
10.1084/jem.185.1.111
发表时间：
1997-01-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Aiuti A;Webb IJ;Bleul C;Springer T;Gutierrez-Ramos JC
通讯作者：
Gutierrez-Ramos JC