Renal macro- and microcirculation autoregulatory capacity during early sepsis and norepinephrine infusion in rats.
Renal macro- and microcirculation autoregulatory capacity during early sepsis and norepinephrine infusion in rats.
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DOI：
10.1186/cc12818
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发表时间：
2013-07-12
期刊：
影响因子：
--
通讯作者：
Lerolle N
中科院分区：
文献类型：
--
作者：
Burban M;Hamel JF;Tabka M;de La Bourdonnaye MR;Duveau A;Mercat A;Calès P;Asfar P;Lerolle N
脓毒症时全身血流动力学与肾血流量和肾微循环的关系知之甚少。去甲肾上腺素（NE）输注可能会增加另一个层次的复杂性。在存在和不存在脓毒症和/或NE的情况下，通过血液去除使通气和麻醉的大鼠经受各种平均动脉压（MAP）步骤。测量肾血流量（RBF）和肾皮质毛细血管中的血流速度（Vm）（使用侧流暗场成像）。使用线性混合模型对数据进行分析，使我们能够显示所考虑的解释变量及其相互作用的影响。MAP与RBF呈正相关。脓毒症对RBF没有独立的影响，而去甲肾上腺素降低RBF，无论是否存在脓毒症。MAP和RBF之间的关系在高于100 mmHg的MAP时比低于100 mmHg时更弱，RBF在高于该阈值时显示出相对的“平台”。脓毒症和NE对颈动脉血流量（CBF）的影响与RBF不同，证明了器官特异性。MAP与Vm呈正相关。脓毒症增加Vm，而nNE降低Vm，与MAP无关。脓毒症与实验结束时测定的血清肌酐增加相关，NE输注可预防脓毒症。在我们的模型中，早期脓毒症在大范围的MAP上不影响RBF。NE引起肾血管收缩作用。在脓毒症中，RBF的自身调节似乎是保守的。相反，脓毒症与NE输注逆转的皮质管周毛细血管血流“超高速”相关。
The relationships between systemic hemodynamics and renal blood flow and renal microcirculation are poorly known in sepsis. Norepinephrine (NE) infusion may add another level of complexity. Ventilated and anesthetized rats were submitted to various mean arterial pressure (MAP) steps by blood removal, in presence and absence of sepsis and/or NE. Renal blood flow (RBF) and blood velocity (Vm) in renal cortical capillaries (using Sidestream Dark Field Imaging) were measured. Data were analyzed using linear mixed models enabling us to display the effects of both the considered explanatory variables and their interactions. Positive correlations were found between MAP and RBF. Sepsis had no independent impact on RBF whereas norepinephrine decreased RBF, regardless of the presence of sepsis. The relationship between MAP and RBF was weaker above a MAP of 100 mmHg as opposed to below 100 mmHg, with RBF displaying a relative "plateau" above this threshold. Sepsis and NE impacted carotid blood flow (CBF) differently compared to RBF, demonstrating organ specificity. A positive relationship was observed between MAP and Vm. Sepsis increased Vm while nNE decreased Vm irrespective of MAP. Sepsis was associated with an increase in serum creatinine determined at the end of the experiments, which was prevented by NE infusion. In our model, sepsis at an early phase did not impact RBF over a large range of MAP. NE elicited a renal vasoconstrictive effect. Autoregulation of RBF appeared conserved in sepsis. Conversely, sepsis was associated with "hypervelocity" of blood flow in cortical peritubular capillaries reversed by NE infusion.
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DOI：
10.1186/cc4948
发表时间：
2006
期刊：
Critical care (London, England)
影响因子：
--
作者：
Johannes T;Mik EG;Nohé B;Raat NJ;Unertl KE;Ince C
通讯作者：
Ince C
影响因子：
3.7
作者：
Payen D;Lukaszewicz AC;Legrand M;Gayat E;Faivre V;Megarbane B;Azoulay E;Fieux F;Charron D;Loiseau P;Busson M
通讯作者：
Busson M
影响因子：
8.8
作者：
Di Giantomasso, D;Morimatsu, H;Bellomo, R
通讯作者：
Bellomo, R
影响因子：
3.8
作者：
Goedhart, P. T.;Khalilzada, M.;Ince, C.
通讯作者：
Ince, C.
影响因子：
15.3
作者：
Mairey, Emilie;Genovesio, Auguste;Dumenil, Guillaume
通讯作者：
Dumenil, Guillaume