Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women
Mitochondrial DNA copy number in cervical exfoliated cells and risk of cervical cancer among HPV-positive women
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HPV阳性女性宫颈脱落细胞线粒体DNA拷贝数与宫颈癌风险
DOI：
10.1186/s12905-020-01001-w
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发表时间：
2019-10
期刊：
影响因子：
--
通讯作者：
Hang Dong
中科院分区：
文献类型：
--
作者：
Sun Wei;Qin Xueyun;Zhou Jing;Xu Mingjing;Lyu Zhangyan;Li Xin;Zhang Kai;Dai Min;Li Ni;Hang Dong
研究背景尽管人类乳头瘤病毒（HPV）感染被认为是99%以上的宫颈癌的病因，但只有一小部分HPV感染的妇女会发展成这种恶性肿瘤。新出现的证据表明，线粒体DNA拷贝数（mtCN）的改变可能有助于癌变。然而，mtCN和宫颈癌之间的关系仍然undetermined.MethodsThe目前的研究包括591例宫颈癌和373例无癌对照，所有这些人都感染了高危型HPV。通过qRT-PCR检测宫颈癌脱落细胞中的相对mtCN，并进行logistic回归分析以计算比值比（OR）和95%置信区间（CI）。结果病例组和对照组中HPV 16、18、52和58型均为最常见的型别。病例组mtCN中位数显著高于对照组（1.63 vs.1.23，P = 0.03）。校正年龄和HPV类型后，与最低四分位数相比，mtCN最高四分位数与宫颈癌发病率增加相关（OR = 1.77，95% CI = 1.19，2.62;P< 0.01）。mtCN对宫颈癌的治疗也有剂量反应效应（P趋势< 0.001）。mtCN和HPV类型之间的相互作用是统计学nonsignificant.ConclusionsIn妇女谁测试HPV阳性，宫颈脱落细胞中的mtCN的增加与宫颈癌。这表明mtCN在宫颈癌发生中的潜在作用。
BackgroundAlthough human papillomavirus (HPV) infection has been regarded as the cause of cervical cancer in over 99% of cases, only a small fraction of HPV-infected women develop this malignancy. Emerging evidence suggests that alterations of mitochondrial DNA copy number (mtCN) may contribute to carcinogenesis. However, the relationship between mtCN and cervical cancer remains undetermined.MethodsThe current study included 591 cervical cancer cases and 373 cancer-free controls, all of whom were infected with high-risk HPV. Relative mtCN in cervical cancer exfoliated cells was measured by qRT-PCR assays, and logistic regression analysis was performed to compute odds ratios (ORs) and 95% confidence intervals (CIs). Interaction between mtCN and HPV types was assessed by using the Wald test in logistic regression models.ResultsHPV16, 18, 52, and 58 were the most common types in both case and control groups. Median mtCN in cases was significantly higher than that in controls (1.63 vs. 1.23,P= 0.03). After adjustment for age and HPV types, the highest quartile of mtCN was associated with increased odds of having cervical cancer (OR = 1.77, 95% CI = 1.19, 2.62;P< 0.01), as compared to the lowest quartile. A dose-response effect of mtCN on cervical cancer was also observed (Ptrend< 0.001). The interaction between mtCN and HPV types was statistically nonsignificant.ConclusionsIn women who test HPV positive, the increase of mtCN in cervical exfoliated cells is associated with cervical cancer. This suggests a potential role of mtCN in cervical carcinogenesis.
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DOI：
10.1007/s11515-014-1332-0
发表时间：
2014-12
期刊：
Frontiers in biology
影响因子：
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