Association of decreased mitochondrial DNA content with the progression of colorectal cancer.
Association of decreased mitochondrial DNA content with the progression of colorectal cancer.
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DOI：
10.1186/1471-2407-13-110
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发表时间：
2013-03-12
期刊：
影响因子：
3.8
通讯作者：
Guo X
中科院分区：
文献类型：
--
作者：
Cui H;Huang P;Wang Z;Zhang Y;Zhang Z;Xu W;Wang X;Han Y;Guo X
实验数据表明，线粒体参与肿瘤发生。然而，对大肠癌组织中mtDNA的定性和定量变化知之甚少。因此，我们进行了可能的相关性线粒体DNA（mtDNA）拷贝数在结直肠癌（CRC）的临床和病理结果和CRC预后。采用实时定量聚合酶链反应分析方法对60例大肠癌患者癌组织及癌旁组织中mtDNA拷贝数进行检测。我们研究了结直肠癌患者mtDNA拷贝数与临床病理参数的相关性。分析mtDNA拷贝数与3年生存率的相关性。结直肠癌组织中mtDNA拷贝数（108.60 ± 20.11）低于相应的非癌组织（153.68 ± 25.72），且与淋巴结转移显著相关。通过Kaplan-Meier曲线评估，mtDNA拷贝数较低的患者的3年生存率往往低于mtDNA拷贝数较高的患者，但相关性不显著（总生存率，63.0 vs 83%）。这些结果表明，mtDNA拷贝数减少与CRC的恶性潜能相关。
Experimental data suggest that mitochondria is involved in tumorigenesis. However, little is known about the qualitative and quantitative changes of mtDNA in colorectal cancer tissues. We therefore conducted possible correlations of the mitochondrial DNA (mtDNA) copy number in colorectal cancer (CRC) with clinical and pathological findings and CRC prognosis. mtDNA copy numbers in CRC cancer tissue and adjacent non-cancerous tissue samples were measured using quantitative real-time polymerase chain reaction analyses from 60 patients admitted to our hospital. We examined the correlation of mtDNA copy numbers and clinicopathologic parameters of CRC patients. The correlation between mtDNA copy number and three-year survival was analyzed. The mtDNA copy number was lower in CRC tissue compared with the corresponding non-cancerous colorectal tissue (mean: 108.60 ± 20.11 vs. 153.68 ± 25.72) and was significantly correlated with lymph-node metastasis. Patients with a lower mtDNA copy number tended to have lower 3-year survival than patients with a higher mtDNA copy number assessed by Kaplan–Meier curves, but the correlation was not significant (overall survival, 63.0 vs 83%). These results suggest that a reduced copy number of mtDNA is correlated with malignant potential in CRC.
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DOI：
10.1007/978-1-59745-416-2_15
发表时间：
2009
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Singh, Keshav K;Kulawiec, Mariola
通讯作者：
Kulawiec, Mariola
影响因子：
3.9
作者：
Gredilla, Ricardo;Bohr, Vilhelm A.;Stevnsner, Tinna
通讯作者：
Stevnsner, Tinna
影响因子：
12.8
作者：
Lee, Wan;Choi, Hyo-Im;Park, Seung-Yoon
通讯作者：
Park, Seung-Yoon
影响因子：
3
作者：
Cook, Cody C.;Higuchi, Masahiro
通讯作者：
Higuchi, Masahiro
影响因子：
--
作者：
Jeon, Jae-Pil;Shim, Sung-Mi;Han, Bok-Ghee
通讯作者：
Han, Bok-Ghee