Differential Effects of Physical and Psychological Stressors on Immune Functions of Rats
Differential Effects of Physical and Psychological Stressors on Immune Functions of Rats
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生理和心理应激源对大鼠免疫功能的不同影响
DOI：
10.1080/1025389031000101330
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发表时间：
2003
期刊：
影响因子：
2.3
通讯作者：
K. Machida
中科院分区：
文献类型：
--
作者：
K. Oishi;N. Nishio;Katsunori Konishi;Masahiko Shimokawa;T. Okuda;T. Kuriyama;K. Machida
为了研究不同类型或时间的应激源对雄性Fischer大鼠免疫功能的影响，在通讯箱中暴露慢性生理（足电）或心理（非足电）应激。体外研究了肺泡巨噬细胞（AMs）产生超氧化物、丝裂原诱导的脾淋巴细胞增殖和脾自然杀伤细胞（NK）的细胞溶解。重复暴露于物理应激下可抑制AMs的超氧化物生成（m为58%，p <0.05, opsonized zymosan （OZ)）和m为51%，p <0.05， phorbol 12-肉豆酸13-乙酸酯（PMA）），但重复暴露24 h后，心理应激可抑制AMs的超氧化物生成（m为40%，p <0.05， OZ和m为47%，p <0.05）。脾淋巴细胞增生反应的急性抑制仅在生理应激组出现（p <0.05），而慢性抑制仅在心理应激组出现（p <0.05）。急性生理应激后NK细胞活性立即受到抑制（p <0.05），而心理应激组对NK细胞活性无影响。这些结果强调了在检查压力对免疫功能的影响时区分生理和心理压力源的重要性。
To study the effects of different types or durations of stressors on immune functions, male Fischer rats were exposed to chronic physical (electric foot shock) or psychological (non-foot shock) stress induced in the communication box. Superoxide production by alveolar macrophages (AMs), mitogen-induced splenic lymphocyte proliferation, and splenic natural killer (NK) cell cytolysis were examined in vitro. Repeated exposure to physical stress suppressed superoxide production by AMs ( m 58%, p <0.05 for opsonized zymosan (OZ) and m 51%, p <0.05 for phorbol 12-myristate 13-acetate (PMA)), although psychological stress suppressed superoxide production after 24 h of repeated exposures ( m 40%, p <0.05 for OZ and m 47%, p <0.05 for PMA). Acute suppression of the blastic response of splenic lymphocytes was only found in the physical stress group ( p <0.05), although the chronic effects were only found in the psychological stress group ( p <0.05). NK cell activity was suppressed immediately after the acute physical stress ( m 30%, p <0.05 ), but no effects were found in the psychological stress group. These results underline the importance of distinguishing between physical versus psychological stressors when examining the effects of stress on immune functions.
酶标仪测定用于测量调理后的酵母聚糖刺激的中性粒细胞产生的超氧阴离子。
DOI：
--
发表时间：
1995
期刊：
BioTechniques.
影响因子：
--
作者：
Carey,LA;Schuhl,RA;Gee,MH
通讯作者：
Gee,MH
暴露于隔离或水调度后，有丝分裂原对淋巴细胞活化的时间依赖性增强。
影响因子：
6.1
作者：
Jessop,JJ;Gale,K;Bayer,BM
通讯作者：
Bayer,BM