Response of engineered cartilage tissue to biochemical agents as studied by proton magnetic resonance microscopy.
Response of engineered cartilage tissue to biochemical agents as studied by proton magnetic resonance microscopy.
复制标题
通过质子磁共振显微镜研究工程软骨组织对生化制剂的反应。
DOI：
10.1002/1529-0131(200007)43:7
复制
发表时间：
2000
影响因子：
--
通讯作者：
Spencer,RG
中科院分区：
文献类型：
--
作者：
Potter,K;Butler,JJ;Horton,WE;Spencer,RG
目的检验磁共振成像（MRI）结果与软骨基质的生化成分相关的假设，因此可用于评估自然组织发育和生物干预的效果。方法将从第 16 天鸡胚胸骨收获的软骨细胞接种到 MRI 兼容的中空纤维生物反应器中。对 2-4 周内形成的组织进行了生化、组织学和 MRI 的研究。除了自然发育外，还研究了组织对视黄酸、白细胞介素-1β (IL-1β) 和每日服用抗坏血酸的反应。结果组织湿重和干重、糖胺聚糖 (GAG) 含量和胶原蛋白含量均随着发育时间的延长而增加，而组织水合作用减少。与对照组相比，施用视黄酸导致组织湿重、蛋白聚糖含量和细胞数量显着减少，并且水合作用增加。与对照组相比，每日服用抗坏血酸显着增加了组织胶原蛋白含量，而施用 IL-1β 则导致蛋白聚糖含量增加。水质子纵向和横向弛豫率与基质的 GAG 和胶原蛋白浓度以及组织水合密切相关。相反，组织的磁化转移值仅与总胶原蛋白相关。最后，水的自扩散系数与组织水合作用相关。 结论 从无创获得的 MR 图像中导出的参数可用于定量评估生物反应器中生成的软骨组织的成分。我们的结论是，MRI 是一种有前途的方法，可用于评估各种环境下软骨的某些生化特性。
ObjectiveTo test the hypothesis that magnetic resonance imaging (MRI) results correlate with the biochemical composition of cartilage matrix and can therefore be used to evaluate natural tissue development and the effects of biologic interventions.MethodsChondrocytes harvested from day‐16 chick embryo sterna were inoculated into an MRI‐compatible hollow‐fiber bioreactor. The tissue that formed over a period of 2–4 weeks was studied biochemically, histologically, and with MRI. Besides natural development, the response of the tissue to administration of retinoic acid, interleukin‐1β (IL‐1β), and daily dosing with ascorbic acid was studied.ResultsTissue wet and dry weight, glycosaminoglycan (GAG) content, and collagen content all increased with development time, while tissue hydration decreased. The administration of retinoic acid resulted in a significant reduction in tissue wet weight, proteoglycan content, and cell number and an increase in hydration as compared with controls. Daily dosing with ascorbic acid increased tissue collagen content significantly compared with controls, while the administration of IL‐1β resulted in increased proteoglycan content. The water proton longitudinal and transverse relaxation rates correlated well with GAG and collagen concentrations of the matrix as well as with tissue hydration. In contrast, the magnetization transfer value for the tissue correlated only with total collagen. Finally, the self‐diffusion coefficient of water correlated with tissue hydration.ConclusionParameters derived from MR images obtained noninvasively can be used to quantitatively assess the composition of cartilage tissue generated in a bioreactor. We conclude that MRI is a promising modality for the assessment of certain biochemical properties of cartilage in a wide variety of settings.
登录
查看更多内容
影响因子：
3.3
作者：
XIA, Y;FARQUHAR, T;JELINSKI, LW
通讯作者：
JELINSKI, LW
人类关节软骨胶原蛋白堆积与年龄相关的变化。
DOI：
10.1016/0304-4165(94)00134-j
发表时间：
1995
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
E. Wachtel;A. Maroudas;R. Schneiderman
通讯作者：
R. Schneiderman
核磁共振成像中的脉冲梯度自旋回波扩散研究
DOI：
--
发表时间：
1990
期刊：
影响因子：
--
作者：
M. Neeman;J. Freyer;L. Sillerud
通讯作者：
L. Sillerud
影响因子：
19.7
作者：
Seo, GS;Aoki, J;Katsuyama, T
通讯作者：
Katsuyama, T
抗坏血酸对培养鸡胚软骨细胞生长和基质成分合成的影响。
DOI：
10.1002/jez.1402000308
发表时间：
1977
期刊：
The Journal of experimental zoology
影响因子：
--
作者：
A. Hajek;M. Solursh
通讯作者：
M. Solursh