Expression site silencing and life-cycle progression appear normal in Argonaute1-deficient Trypanosoma brucei.
Expression site silencing and life-cycle progression appear normal in Argonaute1-deficient Trypanosoma brucei.
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DOI：
10.1016/j.molbiopara.2006.04.005
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发表时间：
2006-09
影响因子：
1.5
通讯作者：
Ullu, Elisabetta
中科院分区：
文献类型：
--
作者：
Janzen, Christian J.;van Deursen, Frederick;Shi, Huafang;Cross, George A. M.;Matthews, Keith R.;Ullu, Elisabetta
近年来，真核生物学的重大突破之一是发现了RNA干扰（RNAi）途径[1]，这是一种在转录和转录后水平上发挥作用的强大基因沉默机制。 RNAi 途径最初被发现是双链 RNA (dsRNA) 触发同源转录物降解的机制，它对于多种基因沉默现象至关重要 [2]，包括异染色质形成、DNA 和组蛋白甲基化、四膜虫中大核 DNA 消除、植物中启动子沉默以及微小 RNA 的翻译控制。此外，在某些生物体中，RNAi 似乎提供或已被提议提供基因组防御机制，以限制移动元件的传播 [3-9]，而在植物中，它是一种强大的抗病毒反应 [10]。在基因水平上，有两种蛋白质是 RNAi 途径的普遍标志：Dicer（一种 RNAse III 相关酶）和 Argonaute 蛋白质家族的成员 [2]。在由长 dsRNA 触发的“经典”RNAi 途径中，Dicer [11] 是将 dsRNA 加工成 20-30 nt 小干扰 RNA (siRNA) 的核酸内切酶，而 Argonaute 家族成员则提供核酸内切酶活性或“切片器”，在与互补 siRNA 碱基配对后切割转录本 [12-16]。布氏锥虫是其中之一。第一个发现 RNAi 途径的生物体 [17]。在哺乳动物的血液中，锥虫以形态上细长的形式增殖，随着寄生虫血症的增加，它们通常会发育成不可分裂的短截形式，这被认为是预先适应了在昆虫载体（舌蝇属，采采蝇）中的生存[18]。血流形式 (BS) 的表面覆盖着一层致密的涂层
In recent years, one of the major breakthroughs in eukaryotic biology has been the discovery of the RNA interference (RNAi) pathway [1], a powerful gene silencing mechanism that operates both at the transcriptional and post-transcriptional levels. Originally discovered as a mechanism through which double-stranded RNA (dsRNA) triggers degradation of homologous transcripts, the RNAi pathway is essential for a variety of gene silencing phenomena [2], including heterochromatin formation, DNA and histone methylation, macronuclear DNA elimination in Tetrahymena, promoter silencing in plants, and translational control by micro RNAs. Furthermore, in certain organisms RNAi appears to provide or has been proposed to provide a genome defense mechanism to limit the spreading of mobile elements [3–9] and in plants it is a powerful antiviral response [10]. At the genetic level there are two proteins that are the universal hallmark of the RNAi pathway: Dicer, an RNAse III-related enzyme, and a member of the Argonaute protein family [2]. In the ‘classical’RNAi pathway, which is triggered by long dsRNAs, Dicer [11] is the endonuclease that processes dsRNA into 20–30 nt small interfering RNAs (siRNAs), whereas an Argonaute family member provides the endonuclease activity or “Slicer” that cleaves transcripts after base-pairing with complementary siRNAs [12–16].Trypanosoma brucei was one of the first organisms where the RNAi pathway was found [17]. In the bloodstream of mammals, trypanosomes proliferate as morphologically slender forms and, as the parasitemia increases, they normally develop into nondividing stumpy forms, which are thought to be pre-adapted for survival in the insect vector (Glossina sspp., the Tsetse)[18]. The surface of bloodstream forms (BS) is covered by a dense coat of a
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影响因子：
64.5
作者：
HORN, D;CROSS, GAM
通讯作者：
CROSS, GAM
影响因子：
10.5
作者：
Miyoshi, K;Tsukumo, H;Siomi, MC
通讯作者：
Siomi, MC
DOI：
10.1083/jcb.125.5.1147
发表时间：
1994-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Matthews KR;Gull K
通讯作者：
Gull K
DOI：
10.1073/pnas.95.25.14687
发表时间：
1998-12-08
影响因子：
11.1
作者：
Ngô, H;Tschudi, C;Ullu, E
通讯作者：
Ullu, E
影响因子：
56.9
作者：
Liu, JD;Carmell, MA;Hannon, GJ
通讯作者：
Hannon, GJ