An ATP-dependent protease homolog ensures basic standards of survival and pathogenicity for Magnaporthe oryzae
An ATP-dependent protease homolog ensures basic standards of survival and pathogenicity for Magnaporthe oryzae
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ATP 依赖性蛋白酶同系物确保了 Magnaporthe oryzae 的生存和致病性的基本标准
DOI：
10.1007/s10658-014-0572-9
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发表时间：
2015-04
影响因子：
1.8
通讯作者：
Shi-Hong Zhang
中科院分区：
文献类型：
--
作者：
Yi Wei;Jinliang Liu;Fucheng Lin;Shi-Hong Zhang
农作物真菌病害的发生需要一个易感寄主、一个强毒的小种和一个有利的环境。在宿主-病原体相互作用中，环境因素起着至关重要的作用，并最终决定疾病的流行。然而，真菌如何应对环境的分子基础仍需要进一步研究。在这里，我们描述了一种损伤受限突变体，其中一个依赖于magnaporthetp的蛋白酶同源物（MAP1）被T-DNA插入破坏。基因缺失和互补转化体（ΔMAP1， ΔMAP1/MAP1）被遗传创建并进行功能分析。在最佳条件下，ΔMAP1未发现明显的表型变异；但在特定胁迫下，ΔMAP1与野生型（或ΔMAP1/MAP1）的营养生长出现显著差异，清楚地反映了MAP1与抗逆性之间的因果关系。MAP1蛋白亚细胞定位于线粒体。当受到氧化应激时，ΔMAP1线粒体比野生型更容易受到损伤。结合ΔMAP1毒力的降低，我们认为MAP1是植物侵染和多逆境抗性所必需的保护因子。综上所述，MAP1突变暗示了田间病害发展的基本要求和对作物保护的潜在影响。
The occurrence of fungal disease of crops requires a susceptible host, a virulent race, and a favourable environment. Environmental factors play crucial roles and ultimately determine epidemic of disease in host-pathogen interactions. However, the molecular basis of how fungi cope with environments still needs further research. Here, we characterized a lesion-restricted mutant of Magnaporthe oryzae, in which a MagnaportheATP-dependent Protease homolog (MAP1) was disrupted by a T-DNA insertion. The gene deletion and complementation transformants (ΔMAP1, ΔMAP1/MAP1) were genetically created and functionally analyzed. Under optimum conditions, no visible phenotypic variations were found in ΔMAP1; but under selected stresses, the significant difference in vegetative growth between ΔMAP1 and wild type (or ΔMAP1/MAP1) emerged, clearly reflecting a causal relationship between MAP1 and stress resistance. The MAP1 protein was subcellularly localized in mitochondria. When subjected to oxidative stress, ΔMAP1 mitochondria were more susceptible to damage than that of the wild type. Linking the decreased virulence in ΔMAP1, we thus propose MAP1, as a protective factor, is required for plant infection and multi-stress resistance. Above all, the MAP1 mutation implies a basic requirement for disease development in the field and a potential implication in crop protection.
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影响因子：
6.7
作者：
Coleman JL;Katona LI;Kuhlow C;Toledo A;Okan NA;Tokarz R;Benach JL
通讯作者：
Benach JL
通过ATP依赖性LON蛋白酶在线粒体中的多任务。
DOI：
10.1016/j.bbamcr.2011.11.003
发表时间：
2012-01
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Venkatesh S;Lee J;Singh K;Lee I;Suzuki CK
通讯作者：
Suzuki CK
影响因子：
6.7
作者：
Huang K;Czymmek KJ;Caplan JL;Sweigard JA;Donofrio NM
通讯作者：
Donofrio NM
影响因子：
3.3
作者：
N. Pagé;Manon Gérard-Vincent;Patrice Ménard;M. Beaulieu;M. Azuma;G. Dijkgraaf;Huijuan Li;José Marcoux;Thuy N Nguyen;Tim Dowse;A. Sdicu;H. Bussey
通讯作者：
N. Pagé;Manon Gérard-Vincent;Patrice Ménard;M. Beaulieu;M. Azuma;G. Dijkgraaf;Huijuan Li;José Marcoux;Thuy N Nguyen;Tim Dowse;A. Sdicu;H. Bussey
影响因子：
3.2
作者：
C. Woldringh;W. Iterson
通讯作者：
C. Woldringh;W. Iterson