Protein synthesis—dependent cytoplasmic translocation of p53 protein after serum stimulation of growth‐arrested MCF‐7 cells
Protein synthesis—dependent cytoplasmic translocation of p53 protein after serum stimulation of growth‐arrested MCF‐7 cells
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生长停滞的 MCF-7 细胞血清刺激后蛋白质合成 - p53 蛋白依赖性胞质易位
DOI：
10.1002/mc.2940080112
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发表时间：
1993
影响因子：
4.6
通讯作者：
T. Ono
中科院分区：
文献类型：
--
作者：
Kazuhide Takahashi;H. Sumimoto;Katsuo Suzuki;T. Ono
p53蛋白定位于生长中的MCF-7细胞的细胞质和生长停滞的MCF-7细胞的细胞核中。虽然生长和停滞细胞中p53合成的绝对量和速率几乎相同，但生长细胞中的蛋白质比停滞细胞中的蛋白质磷酸化程度更大。细胞质和细胞核的p53蛋白结合到DNA序列特异性p53蛋白结合的能力没有显着差异，尽管他们的差异磷酸化水平。放线菌酮抑制血清诱导的p53蛋白从细胞核到细胞质的易位，以及DNA和蛋白质的合成。这些结果表明，DNA合成相关的细胞质易位的p53蛋白在血清刺激依赖于从头蛋白质的合成，而不是改变蛋白质的能力，结合到特定的DNA序列。© 1993 Wiley‐利斯公司
p53 protein was localized in the cytoplasm of growing and in the nucleus of growth‐arrested MCF‐7 cells. While the absolute amount and rate of synthesis of p53 in growing and arrested cells were nearly the same, the protein in growing cells was phosphorylated to a greater extent than in arrested cells. The abilities of the cytoplasmic and nuclear p53 proteins to bind to DNA sequences specific for p53 protein binding did not differ remarkably despite their differential phosphorylation levels. Serum‐induced translocation of the p53 protein from the nucleus to the cytoplasm, as well as DNA and protein synthesis, were inhibited by cycloheximide. These results suggest that the DNA synthesis—associated cytoplasmic translocation of p53 protein in response to serum stimulation depends on de novo protein synthesis and not on alteration of the protein's ability to bind to specific DNA sequences. © 1993 Wiley‐Liss, Inc.
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DOI：
10.1073/pnas.87.5.1973
发表时间：
1990-03-01
影响因子：
11.1
作者：
BRESSAC, B;GALVIN, KM;OZTURK, M
通讯作者：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
56.9
作者：
RAYCROFT, L;WU, H;LOZANO, G
通讯作者：
LOZANO, G
DOI：
10.1073/pnas.87.16.6166
发表时间：
1990-08-01
影响因子：
11.1
作者：
MERCER, WE;SHIELDS, MT;ULLRICH, SJ
通讯作者：
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