Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery.
Histochemistry of microinfarcts in the mouse brain after injection of fluorescent microspheres into the common carotid artery.
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颈总动脉注射荧光微球后小鼠大脑微梗塞的组织化学
DOI：
10.4103/1673-5374.322470
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发表时间：
2022-04
影响因子：
6.1
通讯作者：
Bai WZ
中科院分区：
文献类型：
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作者：
Shen Y;Yao MJ;Su YX;Xu DS;Wang J;Wang GR;Cui JJ;Zhang JL;Bai WZ
通过颈总动脉注射荧光微球建立的小鼠多发性脑梗塞模型是脑缺血动物模型的最新发展。为了研究其有效性，通过将直径 45-53 µm 的荧光微球注射到颈总动脉中来创建脑梗塞小鼠模型。建模六小时后，通过荧光立体显微镜直接观察大脑表面和大脑切片中的荧光微球。使用荧光组织化学和免疫组织化学检查与微梗塞相关的血管、神经元和神经胶质细胞的变化。微球主要分布于注射侧同侧的大脑皮层、纹状体和海马。在存在荧光微球的大脑区域发现了微梗塞。在这里，滞留的微球诱导血管和神经元损伤以及星形胶质细胞和小胶质细胞的激活。这些组织病理学变化表明，这种多发性脑梗塞动物模型有效模拟了在多灶性微梗塞中观察到的各种细胞类型的变化。该模型是研究缺血性中风发病机制的有效附加工具，可用于评估治疗干预措施。本研究于2021年3月16日获得中国中医科学院针灸研究所动物伦理委员会批准（批准号：D2021-03-16-1）。
The mouse model of multiple cerebral infarctions, established by injecting fluorescent microspheres into the common carotid artery, is a recent development in animal models of cerebral ischemia. To investigate its effectiveness, mouse models of cerebral infarction were created by injecting fluorescent microspheres, 45–53 µm in diameter, into the common carotid artery. Six hours after modeling, fluorescent microspheres were observed directly through a fluorescence stereomicroscope, both on the brain surface and in brain sections. Changes in blood vessels, neurons and glial cells associated with microinfarcts were examined using fluorescence histochemistry and immunohistochemistry. The microspheres were distributed mainly in the cerebral cortex, striatum and hippocampus ipsilateral to the side of injection. Microinfarcts were found in the brain regions where the fluorescent microspheres were present. Here the lodged microspheres induced vascular and neuronal injury and the activation of astroglia and microglia. These histopathological changes indicate that this animal model of multiple cerebral infarctions effectively simulates the changes of various cell types observed in multifocal microinfarcts. This model is an effective, additional tool to study the pathogenesis of ischemic stroke and could be used to evaluate therapeutic interventions. This study was approved by the Animal Ethics Committee of the Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences (approval No. D2021-03-16-1) on March 16, 2021.
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免疫细胞，神经胶质和神经元在多发性硬化症中的作用。
影响因子：
6.7
作者：
Mallucci G;Peruzzotti-Jametti L;Bernstock JD;Pluchino S
通讯作者：
Pluchino S
影响因子：
6.7
作者：
Jiang X;Andjelkovic AV;Zhu L;Yang T;Bennett MVL;Chen J;Keep RF;Shi Y
通讯作者：
Shi Y
影响因子：
11.1
作者：
del Zoppo GJ
通讯作者：
del Zoppo GJ
影响因子：
16.2
作者：
Iadecola C
通讯作者：
Iadecola C
影响因子：
25
作者：
通讯作者：
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