Evolution of the casein multigene family: conserved sequences in the 5' flanking and exon regions.
Evolution of the casein multigene family: conserved sequences in the 5' flanking and exon regions.
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酪蛋白多基因家族的进化：5侧翼和外显子区域的保守序列。
DOI：
10.1093/nar/14.4.1883
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发表时间：
1986
影响因子：
14.9
通讯作者：
Rosen,JM
中科院分区：
文献类型：
--
作者：
Yu-Lee,LY;Richter-Mann,L;Couch,CH;Stewart,AF;Mackinlay,AG;Rosen,JM
分离了大鼠α-和牛α-S1-酪蛋白基因，并测定了其5‘端序列。大鼠α-，β-，γ和牛αS1酪蛋白基因含有相似的5‘外显子排列，其中5’非编码、信号肽和酪蛋白KLN酶磷酸化序列分别由不同的外显子编码。这些发现支持一种假设，即在进化过程中，酪蛋白基因家族是通过外显子招募以及原始基因的基因内和基因间复制的过程而产生的。在5‘侧翼DNA的前200个碱基对中，已经确定了几个高度保守的区域。在大鼠α-和牛αS1-酪蛋白序列之间还发现了CAP位点上游长达550个碱基对的额外序列同源性。出人意料的是，这些基因的5‘侧翼启动子区域比这些基因的整个成熟编码区和内含子区域都更保守。这些保守的5‘侧翼序列可能含有潜在的顺式调控元件，这些元件负责在乳腺发育过程中协调表达与功能相关的酪蛋白基因。
The rat α- and bovine αs1-casein genes have been isolated and their 5′ sequences determined. The rat α-, β-, γ- and bovine αs1-casein genes contain similar 5′ exon arrangements in which the 5′ noncoding, signal peptide and casein klnase phosphorylatlon sequences are each encoded by separate exons. These findings support the hypothesis that during evolution, the family of casein genes arose by a process involving exon recruitment followed by intra-genic and intergenic duplication of a primordial gene. Several highly conserved regions in the first 200 base pairs of the 5′ flanking DNA have been identified. Additional sequence homology extending up to 550 base pairs upstream of the CAP site has been found between the rat α- and bovine αs1-casein sequences. Unexpectedly, the 5′ flanking promoter regions are conserved to a greater extent than both the entire mature coding and intron regions of these genes. These conserved 5′ flanking sequences may contain potential cis regulatory elements which are responsible for the coordinate expression of the functionally-related casein genes during mammary gland development.
糖皮质激素和孕激素受体结合到两个激素调节启动子的相同位点
影响因子：
64.8
作者：
D. V. D. Ahe;S. Janich;C. Scheidereit;R. Renkawitz;G. Schütz;M. Beato
通讯作者：
M. Beato
基因组 Poly(dT-dG).poly(dC-dA) 序列的表征：结构、组织和构象
影响因子：
5.3
作者：
H. Hamada;M. Petrino;T. Kakunaga;M. Seidman;B. Stollar
通讯作者：
B. Stollar