Nisin and Nisin Probiotic Disrupt Oral Pathogenic Biofilms and Restore Their Microbiome Composition towards Healthy Control Levels in a Peri-Implantitis Setting.
Nisin and Nisin Probiotic Disrupt Oral Pathogenic Biofilms and Restore Their Microbiome Composition towards Healthy Control Levels in a Peri-Implantitis Setting.
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Nisin和Nisin益生菌破坏口腔病原体生物膜，并在种植体周围环境中将其微生物组组成恢复到健康控制水平。
DOI：
10.3390/microorganisms10071336
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发表时间：
2022-07-01
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
种植体周围炎的特征是种植体周围支持组织的慢性炎症，这种炎症会逐渐且不可逆转地导致骨丢失，进而导致种植体丢失。与牙周疾病类似，口腔菌群失调被认为是种植体周围炎的一个诱因。然而，使用传统治疗方法，如非手术清创或手术来治疗种植体周围炎并不总是成功的。因此，人们提出了新的策略来解决这些缺陷。一种策略是使用益生菌作为抗菌剂，因为它们被认为对人类和环境是安全的。具体而言，益生菌乳酸乳球菌能产生乳链菌肽，这种物质在全球范围内被用于食品保鲜。本研究的目的是确定乳链菌肽以及产生乳链菌肽的野生型（WT）乳酸乳球菌益生菌是否能够破坏口腔致病性生物膜，并在钛片上的这些口腔生物膜内促进更健康的口腔微生物群落。通过共聚焦成像和16S rRNA测序，本研究表明乳链菌肽和野生型乳酸乳球菌益生菌在体外种植体周围炎环境中能够破坏口腔致病性生物膜。更具体地说，乳链菌肽使添加病原体的生物膜的活力呈剂量依赖性地从62.53±3.69%分别降低到54.26±3.35%和44.88±2.98%。同样，10⁵菌落形成单位/毫升的野生型乳酸乳球菌显著将生物膜活力降低到52.45±3.41%。此外，两种处理都使这些生物膜的组成、相对丰度和多样性水平朝着健康对照水平转变。总计1微克/毫升的乳链菌肽和10³菌落形成单位/毫升的野生型乳酸乳球菌能够使变形菌门（从80.5±2.9%分别恢复到75.6±2.0%、78.0±2.8%和75.1±5.3%）和厚壁菌门（从11.6±1.6%分别恢复到15.4±1.3%、13.8±1.8%和13.7±2.6%）中病原体介导的变化恢复到对照水平。因此，乳链菌肽及其产生乳链菌肽的乳酸乳球菌益生菌可能通过促进更健康的口腔生物膜对治疗种植体周围炎有用，这可能有助于改善患者的口腔健康。
Peri-implantitis is characterized by chronic inflammation of the peri-implant supporting tissues that progressively and irreversibly leads to bone loss and, consequently, implant loss. Similar to periodontal disease, oral dysbiosis is thought to be a driver of peri-implantitis. However, managing peri-implantitis with traditional treatment methods, such as nonsurgical debridement or surgery, is not always successful. Thus, novel strategies have been proposed to address these shortcomings. One strategy is the use of probiotics as antimicrobial agents since they are considered safe for humans and the environment. Specifically, the probiotic Lactococcus lactis produces nisin, which has been used worldwide for food preservation. The objective of this study was to determine whether nisin and the wild-type (WT) nisin-producing L. lactis probiotic can disrupt oral pathogenic biofilms and promote a healthier oral microbiome within these oral biofilms on titanium discs. Using confocal imaging and 16S rRNA sequencing, this study revealed that nisin and WT L. lactis probiotic disrupt oral pathogenic biofilms in a peri-implantitis setting in vitro. More specifically, nisin decreased the viability of the pathogen-spiked biofilms dose-dependently from 62.53 ± 3.69% to 54.26 ± 3.35% and 44.88 ± 2.98%, respectively. Similarly, 105 CFU/mL of WT L. lactis significantly decreased biofilm viability to 52.45 ± 3.41%. Further, both treatments shift the composition, relative abundance, and diversity levels of these biofilms towards healthy control levels. A total of 1 µg/mL of nisin and 103 CFU/mL of WT L. lactis were able to revert the pathogen-mediated changes in the Proteobacteria (from 80.5 ± 2.9% to 75.6 ± 2.0%, 78.0 ± 2.8%, and 75.1 ± 5.3%, respectively) and Firmicutes (from 11.6 ± 1.6% to 15.4 ± 1.3%, 13.8 ± 1.8%, and 13.7 ± 2.6%, respectively) phyla back towards control levels. Thus, nisin and its nisin-producing L. lactis probiotic may be useful in treating peri-implantitis by promoting healthier oral biofilms, which may be useful for improving patient oral health.
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DOI：
10.2174/1874210601408010050
发表时间：
2014
期刊：
The open dentistry journal
影响因子：
--
作者：
Abraham CM
通讯作者：
Abraham CM
影响因子：
6.2
作者：
Limaye, Sewanti Atul;Haddad, Robert I.;Murphy, Barbara A.
通讯作者：
Murphy, Barbara A.
使用通用的unifrac距离将微生物组组成与环境协变量相关联。
DOI：
10.1093/bioinformatics/bts342
发表时间：
2012-08-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Chen J;Bittinger K;Charlson ES;Hoffmann C;Lewis J;Wu GD;Collman RG;Bushman FD;Li H
通讯作者：
Li H
DOI：
10.2147/ccide.s31005
发表时间：
2013
期刊：
Clinical, cosmetic and investigational dentistry
影响因子：
--
作者：
Do T;Devine D;Marsh PD
通讯作者：
Marsh PD
影响因子：
4.6
作者：
Lin X;Xu J;Shi Z;Xu Y;Fu T;Zhang L;He F
通讯作者：
He F