Dynamin2- and endothelial nitric oxide synthase-regulated invasion of bladder epithelial cells by uropathogenic Escherichia coli.
Dynamin2- and endothelial nitric oxide synthase-regulated invasion of bladder epithelial cells by uropathogenic Escherichia coli.
复制标题
DOI：
10.1083/jcb.201003027
复制
发表时间：
2011-01-10
期刊：
影响因子：
--
通讯作者：
Daaka Y
中科院分区：
文献类型：
--
作者：
Wang Z;Humphrey C;Frilot N;Wang G;Nie Z;Moniri NH;Daaka Y
eNOS-mediated S-nitrosylation of dynamin2 promotes infection of epithelial cells by E. coli. Invasion of bladder epithelial cells by uropathogenic Escherichia coli (UPEC) contributes to antibiotic-resistant and recurrent urinary tract infections (UTIs), but this process is incompletely understood. In this paper, we provide evidence that the large guanosine triphosphatase dynamin2 and its partner, endothelial nitric oxide (NO) synthase (NOS [eNOS]), mediate bacterial entry. Overexpression of dynamin2 or treatment with the NO donor S-nitrosothiols increases, whereas targeted reduction of endogenous dynamin2 or eNOS expression with ribonucleic acid interference impairs, bacterial invasion. Exposure of mouse bladder to small molecule NOS inhibitors abrogates infection of the uroepithelium by E. coli, and, concordantly, bacteria more efficiently invade uroepithelia isolated from wild-type compared with eNOS−/− mice. E. coli internalization promotes rapid phosphorylation of host cell eNOS and NO generation, and dynamin2 S-nitrosylation, a posttranslational modification required for the bacterial entry, also increases during E. coli invasion. These findings suggest that UPEC escape urinary flushing and immune cell surveillance by means of eNOS-dependent dynamin2 S-nitrosylation and invasion of host cells to cause recurrent UTIs.
登录
查看更多内容
影响因子：
3.4
作者：
Eto, Danelle S.;Gordon, Hannah B.;Dhakal, Bijaya K.
通讯作者：
Dhakal, Bijaya K.
影响因子：
4.8
作者：
Ahn, S;Maudsley, S;Daaka, Y
通讯作者：
Daaka, Y
影响因子：
4
作者：
Kang-Decker, Ningling;Cao, Sheng;Shah, Vijay
通讯作者：
Shah, Vijay
影响因子：
3.1
作者：
Mulvey, MA;Schilling, JD;Hultgren, SJ
通讯作者：
Hultgren, SJ
影响因子：
4.8
作者：
Duncan, MJ;Li, GJ;Abraham, SN
通讯作者：
Abraham, SN