Proteomic analysis reveals rattlesnake venom modulation of proteins associated with cardiac tissue damage in mouse hearts.
Proteomic analysis reveals rattlesnake venom modulation of proteins associated with cardiac tissue damage in mouse hearts.
复制标题
DOI：
10.1016/j.jprot.2022.104530
复制
发表时间：
2022-04-30
影响因子：
3.3
通讯作者：
Iwai, Leo Kei
中科院分区：
文献类型：
--
作者：
Santos, W. S.;Montoni, Fabio;Eichler, R. A. S.;Suehiro Arcos, Stephanie Santos;Andreotti, Diana Zukas;Kisaki, Carolina Yukiko;Evangelista, Kimberly Borges;Calacina, Hamida Macedo;Lima, Ismael Feitosa;Maltauro Soares, Magna Aparecida;Kyle Gren, Eric Conrad;Carvalho, Valdemir Melechco;Ferro, Emer Suavinho;Nishiyama-Jr, Milton Yutaka;Chen, Zhibin;Iwai, Leo Kei
关键词：
蛇毒是一种常见但被忽视的疾病，每年影响着世界各地数百万人。在巴西的毒蛇物种中，热带响尾蛇(Crotalus Durisss Terriffus)是致命性毒蛇数量最多的物种，也是咬伤次数第二多的物种。蛇毒是一种复杂的分泌物，注射后会引发多种生理效应，可能会导致严重的伤害或死亡。恐怖蛇毒的成分具有神经毒性、肌肉毒性、血液毒性、肾毒性和心脏毒性，临床表现为中枢神经系统功能改变、运动瘫痪、癫痫、眼睑下垂、眼肌麻痹、视力模糊、凝血障碍、横纹肌溶解、肌红蛋白尿和心跳呼吸骤停。在这项研究中，我们在小鼠模型上重点研究了恐怖梭菌毒液的心脏毒性作用的蛋白质组学特征。我们将半数致死量(LD50)的蛇毒注射到腓肠肌。分别于注射蛇毒后1h、6h、12h、24h取小鼠心脏，经胰酶消化后进行高分辨质谱分析。我们分析了~GT；1300个蛋白质的蛋白质组谱，观察到几个蛋白质在其定量谱中显示出显著的变化，可能反映了毒液成分的毒性活性。在受影响的蛋白质中，有几种与细胞调控放松和组织损伤有关。心脏蛋白丰度的变化提供了关于它们如何在毒化时协同工作的见解。
Snake envenomation is a common but neglected disease that affects millions of people around the world annually. Among venomous snake species in Brazil, the tropical rattlesnake (Crotalus durissus terrificus) accounts for the highest number of fatal envenomations and is responsible for the second highest number of bites. Snake venoms are complex secretions which, upon injection, trigger diverse physiological effects that can cause significant injury or death. The components of C. d. terrificus venom exhibit neurotoxic, myotoxic, hemotoxic, nephrotoxic, and cardiotoxic properties which present clinically as alteration of central nervous system function, motor paralysis, seizures, eyelid ptosis, ophthalmoplesia, blurred vision, coagulation disorders, rhabdomyolysis, myoglobinuria, and cardiorespiratory arrest. In this study, we focused on proteomic characterization of the cardiotoxic effects of C. d. terrificus venom in mouse models. We injected venom at half the lethal dose (LD50) into the gastrocnemius muscle. Mouse hearts were removed at set time points after venom injection (1 h, 6 h, 12 h, or 24 h) and subjected to trypsin digestion prior to high-resolution mass spectrometry. We analyzed the proteomic profiles of >1,300 proteins and observed that several proteins showed noteworthy changes in their quantitative profiles, likely reflecting the toxic activity of venom components. Among the affected proteins were several associated with cellular deregulation and tissue damage. Changes in heart protein abundance offer insights into how they may work synergistically upon envenomation.
登录
查看更多内容
影响因子：
10.8
作者：
Chen, Le;Gong, Qizhi;Knowlton, Anne A.
通讯作者：
Knowlton, Anne A.
DOI：
10.1016/0035-9203(88)90044-2
发表时间：
1988-11-01
影响因子：
2.2
作者：
CUPO, P;AZEVEDOMARQUES, MM;HERING, SE
通讯作者：
HERING, SE
影响因子：
3.6
作者：
da Silva, Jonas Golart;Soley, Bruna da Silva;Acco, Alexandra
通讯作者：
Acco, Alexandra
影响因子：
3.3
作者：
de Oliveira, Isadora Sousa;Cardoso, Iara Aime;Arantes, Eliane Candiani
通讯作者：
Arantes, Eliane Candiani
影响因子：
2.8
作者：
AZEVEDOMARQUES, MM;CUPO, P;LAURE, CJ
通讯作者：
LAURE, CJ