Epidemiology of Trypanosoma evansi and Trypanosoma vivax in domestic animals from selected districts of Tigray and Afar regions, Northern Ethiopia.
Epidemiology of Trypanosoma evansi and Trypanosoma vivax in domestic animals from selected districts of Tigray and Afar regions, Northern Ethiopia.
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DOI：
10.1186/s13071-015-0818-1
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发表时间：
2015-04-09
影响因子：
3.2
通讯作者：
Büscher P
中科院分区：
文献类型：
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作者：
Birhanu H;Fikru R;Said M;Kidane W;Gebrehiwot T;Hagos A;Alemu T;Dawit T;Berkvens D;Goddeeris BM;Büscher P
非洲动物锥虫病由采采蝇周期性传播或由其他叮咬蝇机械性传播，对牲畜健康造成严重危害。本研究调查了锥虫属（T.）evansi和T.埃塞俄比亚北方无采采蝇地区、阿法尔和提格雷的家畜中存在间日疟。对754只单峰骆驼、493头牛、264只山羊、181只绵羊、84头驴、25匹马和10头骡子进行了横断面研究。采用微量红细胞压积离心法进行寄生虫学试验。采集血浆用CATT/T.evansi和RoTat 1.2免疫锥虫溶解法（ITL）进行血清学诊断，采集血沉棕黄层标本用T.伊氏A型特异性RoTat 1.2 PCR，T.伊氏B型特异性EVAB PCR和T.间日疟原虫特异性TvPRAC PCR。提格雷和阿法尔的寄生虫患病率分别为4.7%和2.7%，牛（7.3%）显著高于其他宿主（z = 2.53，p = 0.011）。CATT/伊氏锥虫的血清阳性率在提格雷为24.6%，在阿法尔为13.9%，在牛中（37.3%）显著高于其他宿主（z = 9.39，p < 0.001）。ITL检测的阳性率仅为1.9%，提示CATT/伊氏锥虫与伊氏锥虫有交叉反应。间日疟原虫或其他锥虫感染。T.伊氏A型在提格雷州和阿法尔州为8.0%，在马和绵羊中的比例从28.0%到2.2%不等。骆驼（11.7%）中的阳性率也显著高于（p < 0.001）牛（6.1%）、驴（6%）、山羊（3.8%）和绵羊（2.2%）。4头骆驼T.伊氏B型。T.间日疟为3.0%，提格雷和阿法尔的间日疟率相近。除在马和骡体内未检出外，其他宿主间差异不显著。NTTAT由T.间日疟原虫和T.伊文思是提格雷州和阿法尔州动物健康的重要威胁。首次证实了T.埃塞俄比亚骆驼中的伊文思B型。目前在牛中进行的诊断试验获得了无法解释的结果，因此需要特别努力分离和消灭在埃塞俄比亚北方流行的锥虫菌株。
African animal trypanosomosis, transmitted cyclically by tsetse flies or mechanically by other biting flies, causes serious inflictions to livestock health. This study investigates the extent of non-tsetse transmitted animal trypanosomosis (NTTAT) by Trypanosoma (T.) evansi and T. vivax in domestic animals in the tsetse-free regions of Northern Ethiopia, Afar and Tigray. A cross sectional study was conducted on 754 dromedary camels, 493 cattle, 264 goats, 181 sheep, 84 donkeys, 25 horses and 10 mules. The microhaematocrit centrifugation technique was used as parasitological test. Plasma was collected for serodiagnosis with CATT/T.evansi and RoTat 1.2 immune trypanolysis (ITL) while buffy coat specimens were collected for molecular diagnosis with T. evansi type A specific RoTat 1.2 PCR, T. evansi type B specific EVAB PCR and T. vivax specific TvPRAC PCR. The parasitological prevalence was 4.7% in Tigray and 2.7% in Afar and significantly higher (z = 2.53, p = 0.011) in cattle (7.3%) than in the other hosts. Seroprevalence in CATT/T.evansi was 24.6% in Tigray and 13.9% in Afar and was significantly higher (z = 9.39, p < 0.001) in cattle (37.3%) than in the other hosts. On the other hand, seroprevalence assessed by ITL was only 1.9% suggesting cross reaction of CATT/T.evansi with T. vivax or other trypanosome infections. Molecular prevalence of T. evansi type A was 8.0% in Tigray and in Afar and varied from 28.0% in horses to 2.2% in sheep. It was also significantly higher (p < 0.001) in camel (11.7%) than in cattle (6.1%), donkey (6%), goat (3.8%), and sheep (2.2%). Four camels were positive for T. evansi type B. Molecular prevalence of T. vivax was 3.0% and was similar in Tigray and Afar. It didn’t differ significantly among the host species except that it was not detected in horses and mules. NTTAT caused by T. vivax and T. evansi, is an important threat to animal health in Tigray and Afar. For the first time, we confirm the presence of T. evansi type B in Ethiopian camels. Unexplained results obtained with the current diagnostic tests in bovines warrant particular efforts to isolate and characterise trypanosome strains that circulate in Northern Ethiopia.
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影响因子：
2.4
作者：
Cortez, A. P.;Ventura, R. M.;Teixeira, M. M. G.
通讯作者：
Teixeira, M. M. G.
影响因子：
2.6
作者：
Aref, Mohammad;Yasin, Saeed Mohammad;Deborggraeve, Stijn
通讯作者：
Deborggraeve, Stijn
伊氏锥虫和苏拉：关于起源、历史、分布、分类、形态、宿主和致病作用的回顾和观点。
影响因子：
--
作者：
Desquesnes M;Holzmuller P;Lai DH;Dargantes A;Lun ZR;Jittaplapong S
通讯作者：
Jittaplapong S
影响因子：
3.8
作者：
Buescher, Philippe;Ngoyi, Dieudonne Mumba;Bieler, Sylvain
通讯作者：
Bieler, Sylvain
影响因子：
3.3
作者：
Camara, Oumou;Camara, Mamadou;Jamonneau, Vincent
通讯作者：
Jamonneau, Vincent