Comparison of nucleocapsid and spike antibody ELISAs for determining SARS-CoV-2 seropositivity in Kenyan women and infants.
Comparison of nucleocapsid and spike antibody ELISAs for determining SARS-CoV-2 seropositivity in Kenyan women and infants.
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DOI：
10.1002/jmv.28221
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发表时间：
2023-01
影响因子：
12.7
通讯作者：
Chohan, Bhavna H.
中科院分区：
文献类型：
--
作者：
Fish, Carolyn S.;Owiti, Prestone;Begnel, Emily R.;Itell, Hannah L.;Ojee, Ednah;Adhiambo, Judith;Ogweno, Vincent;Holland, LaRinda A.;Richardson, Barbra A.;Khan, Adam K.;Maqsood, Rabia;Gantt, Soren;Lim, Efrem S.;Slyker, Jennifer;Kinuthia, John;Overbaugh, Julie;Wamalwa, Dalton;Lehman, Dara A.;Chohan, Bhavna H.
自2019年底冠状病毒病2019大流行开始以来，已经开发了多种酶联免疫吸附试验（elisa）来检测严重急性呼吸综合征冠状病毒2 （SARS‐CoV‐2）抗体。评估这些检测在全球不同人群中的可靠性至关重要。本研究在肯尼亚人群血清阳性率研究中比较了市售的血小板总抗体测定（Bio - Rad）核衣壳酶联免疫吸附试验与广泛使用的西奈山刺突IgG酶联免疫吸附试验的使用。该研究使用2019年5月至2020年12月期间在肯尼亚内罗毕生活的母婴队列中收集的纵向血浆样本，表明两种检测方法在重复测量中具有很高的定性一致性（92.7%）和很强的抗体水平相关性（r2 = 0.973）。在该队列中，两种ELISA检测到的血清阳性率与之前公布的肯尼亚采样期间的血清阳性率估计值非常相似，两种方法检测到的SARS - CoV - 2抗体的发生率没有显著差异。检测的可比性不受HIV暴露状况的影响。这些数据支持使用血小板SARS - CoV - 2总抗体ELISA作为肯尼亚血清流行率研究的合适高通量方法。
A multitude of enzyme‐linked immunosorbent assays (ELISAs) has been developed to detect severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) antibodies since the coronavirus disease 2019 pandemic started in late 2019. Assessing the reliability of these assays in diverse global populations is critical. This study compares the use of the commercially available Platelia Total Ab Assay (Bio‐Rad) nucleocapsid ELISA to the widely used Mount Sinai spike IgG ELISA in a Kenyan population seroprevalence study. Using longitudinal plasma specimens collected from a mother–infant cohort living in Nairobi, Kenya between May 2019 and December 2020, this study demonstrates that the two assays have a high qualitative agreement (92.7%) and strong correlation of antibody levels (R 2 = 0.973) in repeated measures. Within this cohort, seroprevalence detected by either ELISA closely resembled previously published seroprevalence estimates for Kenya during the sampling period and no significant difference in the incidence of SARS‐CoV‐2 antibody detection by either assay was observed. Assay comparability was not affected by HIV exposure status. These data support the use of the Platelia SARS‐CoV‐2 Total Ab ELISA as a suitable high‐throughput method for seroprevalence studies in Kenya.
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影响因子：
5.8
作者：
Sander I;Kespohl S;Zahradnik E;Göcke P;Hosbach I;Herrmann BL;Brüning T;Raulf M
通讯作者：
Raulf M
DOI：
10.1126/science.abk0414
发表时间：
2021-11-19
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Brand SPC;Ojal J;Aziza R;Were V;Okiro EA;Kombe IK;Mburu C;Ogero M;Agweyu A;Warimwe GM;Nyagwange J;Karanja H;Gitonga JN;Mugo D;Uyoga S;Adetifa IMO;Scott JAG;Otieno E;Murunga N;Otiende M;Ochola-Oyier LI;Agoti CN;Githinji G;Kasera K;Amoth P;Mwangangi M;Aman R;Ng'ang'a W;Tsofa B;Bejon P;Keeling MJ;Nokes DJ;Barasa E
通讯作者：
Barasa E
影响因子：
15.8
作者：
ter Meulen, Jan;van den Brink, Edward N.;Poon, Leo L. M.;Marissen, Wilfred E.;Leung, Cynthia S. W.;Cox, Freek;Cheung, Chung Y.;Bakker, Arjen Q.;Bogaards, Johannes A.;van Deventer, Els;Preiser, Wolfgang;Doerr, Hans Wilhelm;Chow, Vincent T.;de Kruif, John;Peiris, Joseph S. M.;Goudsmit, Jaap
通讯作者：
Goudsmit, Jaap
SARS-CoV-2 蛋白质组微阵列，用于对 COVID-19 特异性 IgG 和 IgM 反应进行全局分析
影响因子：
16.6
作者：
Jiang, He-wei;Li, Yang;Tao, Sheng-ce
通讯作者：
Tao, Sheng-ce
影响因子：
--
作者：
Stadlbauer, Daniel;Amanat, Fatima;Krammer, Florian
通讯作者：
Krammer, Florian