Gene expression profiling of colorectal adenomas and early invasive carcinomas by cDNA array analysis.
Gene expression profiling of colorectal adenomas and early invasive carcinomas by cDNA array analysis.
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DOI：
10.1038/sj.bjc.6602442
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发表时间：
2005-04-11
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
人们普遍认为，大多数结直肠癌发生在预先存在的腺瘤。从形态学上看，结直肠腺瘤可分为突出型和平坦型两类。本研究的目的是阐明相关基因表达的变化与大肠癌发生的早期阶段。应用cDNA微阵列技术，我们分析了36例大肠腺瘤（18例扁平型腺瘤和18例弥漫型腺瘤）和14例早期浸润癌中550个癌相关基因的表达谱。在550个基因中，我们选择了32个基因，与匹配的正常组织相比，这些基因的平均表达水平在肿瘤组织中至少上调或下调三倍。在肿瘤组织中分别鉴定出13个和19个基因为上调和下调基因。在上调的基因中，E1 AF、骨形态发生蛋白（BMP）-4、胰岛素样生长因子（IGF）-2、诱导型一氧化氮合酶（iNOS）、金属蛋白酶组织抑制剂（TIMP）-1、Smad 4和nm 23在肿瘤组织中的平均表达水平比匹配的正常组织中的平均表达水平高5倍以上。结直肠腺瘤和早期浸润癌被分为两个主要集群聚类分析。此外，通过聚类分析将扁平型和扁平型腺瘤分为两大类。通过cDNA阵列获得的表达谱清楚地表明，结直肠腺瘤和早期浸润性癌具有特定的表达谱。同样地，扁平型腺瘤和扁平型腺瘤的基因表达谱也不同。这些结果表明，早期结直肠肿瘤的分子分类的cDNA阵列是可行的。
It is generally accepted that most colorectal carcinomas arise in pre-existing adenomas. Morphologically, colorectal adenomas can be divided into two groups, protruded type and flat type. The aim of this study was to clarify relevant alterations of gene expression associated with the early stage of colorectal carcinogenesis. Using cDNA array, we analysed the expression profiles of 550 cancer-related genes in 36 colorectal adenomas (18 flat-type and 18 protruded-type adenomas) and 14 early invasive carcinomas. Among the 550 genes, we chose 32 genes the average expression levels of which were at least three-fold up- or downregulated in tumour tissues compared with levels in matched normal tissues. A total of 13 and 19 genes were identified as up- and downregulated genes in tumour tissues, respectively. Among the upregulated genes, the average expression levels of E1AF, bone morphogenic protein (BMP)-4, insulin-like growth factor (IGF)-2, inducible nitric oxide synthase (iNOS), tissue inhibitors of metalloproteinase (TIMP)-1, Smad4, and nm23 in tumour tissues were over five times higher than those in matched normal tissues. Colorectal adenomas and early invasive carcinomas were divided into two major clusters by clustering analysis. Moreover, flat- and protruded-type adenomas were divided into two major clusters by clustering analysis. The expression profiles obtained by the cDNA array clearly indicate that colorectal adenomas and early invasive carcinomas have specific expression profiles. Likewise, the gene expression profiles of flat- and protruded-type adenomas are different. These results indicate that molecular classification of early colorectal tumours by a cDNA array is feasible.
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影响因子：
3.5
作者：
Kawamoto, K;Onodera, H;Imamura, M
通讯作者：
Imamura, M
影响因子：
8
作者：
Lin, YM;Furukawa, Y;Nakamura, Y
通讯作者：
Nakamura, Y
DOI：
10.1073/pnas.92.10.4392
发表时间：
1995-05-09
影响因子：
11.1
作者：
JENKINS, DC;CHARLES, IG;MONCADA, S
通讯作者：
MONCADA, S
影响因子：
8
作者：
Müller, N;Reinacher-Schick, A;Schwarte-Waldhoff, I
通讯作者：
Schwarte-Waldhoff, I
影响因子：
4.8
作者：
Liu, YM;Borchert, GL;Phang, JM
通讯作者：
Phang, JM