Renal glomerular and tubular injury after gastric bypass in obese rats.
Renal glomerular and tubular injury after gastric bypass in obese rats.
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DOI：
10.1016/j.nut.2011.03.003
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Meguid MM
中科院分区：
文献类型：
--
作者：
Canales BK;Reyes L;Reinhard MK;Khan SR;Goncalves CG;Meguid MM
Roux-en-Y胃分流术(RYGB)是病态肥胖患者长期减肥的最常见的外科干预措施。通过减少肥胖相关的高滤过、糖尿病和高血压，RYGB被吹捧为稳定如果不是预防慢性肾脏疾病的进展。为了验证这一点，我们将接受RYGB手术的饮食诱导肥胖大鼠的肾脏组织学与配对喂养和假肥胖对照组进行了比较。SD大鼠喂饲高脂低草酸饮食诱导肥胖，随机分为RYGB(n=6)、GI完整假手术肥胖对照组(Sham，n=4)和GI完整假手术肥胖成对喂养大鼠(FED，n=8)。记录每日体重和食物摄入量。术后第42天取材，行肾组织学和免疫组织化学检查。肾脏病理通过有经验的兽医病理学家的肾小球损害分类评分和肾小球/肾小管定量评分系统进行评估。骨桥蛋白(OPN)和ED-1(单核/巨噬细胞)染色分别以染色面积百分比和计数细胞数/高倍视野为指标。与Sham和Feed对照组相比，RYGB大鼠的体重显著减轻(p<；0.001)，肾小球病变明显增多(p=0.02)，肾小球和肾小管病变评分也较高(p<；0.01)。与假手术组和饲喂对照组相比，RYGB大鼠在内髓区(P<；0.005)和外髓区(P<；0.02)的骨桥蛋白和ED-1的表达明显增强。在这个饮食诱导的肥胖大鼠模型中，RYGB与慢性肾小球硬化和肾小管间质肾炎有关，组织学和免疫组织化学证实。计划进行前瞻性研究，以更好地确定该模型中的损伤机制。
Roux-en-Y gastric bypass (RYGB) surgery is the most common surgical intervention for long-term weight loss in morbidly obese patients. By reducing obesity-associated hyperfiltration, diabetes, and hypertension, RYGB is touted to stabilize if not prevent progression of chronic renal disease. To test this, we compare renal histology of diet-induced obese rats that have undergone RYGB surgery to pair-fed and sham obese controls. Sprague-Dawley rats, fed a high fat, low-oxalate diet to induce gross obesity, were randomized to RYGB (n=6), GI-intact sham-operated obese controls (Sham, n=4), or GI-intact sham-operated obese pair-fed rats (Fed, n=8). Daily body weight and food intake were recorded. On post-operative day 42, renal histology and immunohistochemistry was examined. Renal pathology was assessed by a categorical glomerular lesion score and a quantitative glomerular/tubular scoring system by experienced veterinary pathologists. Osteopontin (OPN) and ED-1 (monocyte/macrophage cell) staining were estimated on percentage of stained area and number of counted cells/high power field respectively. Compared to sham and fed controls, RYGB rats had significant reductions in body weight (p<0.001), more glomerular lesions (p=0.02), and received higher glomerular and tubular lesion scores (p<0.01). RYGB rodents had significantly stronger staining for OPN within the inner medullary region (p<0.005) and ED-1 within the outer medullary region (P<0.02) compared to sham and fed controls. In this diet-induced obese rat model, RYGB is associated with chronic glomerulosclerosis and tubulointerstitial nephritis, confirmed by histology and immunohistochemistry. Prospective studies to better define the injurious mechanisms in this model are planned.
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影响因子：
6.6
作者：
Asplin, John R.;Coe, Fredric L.
通讯作者：
Coe, Fredric L.
DOI：
10.1016/j.soard.2005.07.002
发表时间：
2005-09-01
期刊：
Surgery for obesity and related diseases : official journal of the American Society for Bariatric Surgery
影响因子：
--
作者：
Nelson, Wayne K;Houghton, Scott G;Sarr, Michael G
通讯作者：
Sarr, Michael G
影响因子：
19.6
作者：
Sinha, M. K.;Collazo-Clavell, M. L.;Lieske, J. C.
通讯作者：
Lieske, J. C.
影响因子：
2.2
作者：
Xu, Y;Ohinata, K;Inui, A
通讯作者：
Inui, A
DOI：
10.1152/ajpregu.00171.2007
发表时间：
2007-10-01
影响因子：
2.8
作者：
Guijarro, Ana;Suzuki, Susumu;Meguid, Michael M.
通讯作者：
Meguid, Michael M.