Whole-genome sequencing reveals transmission of gonococcal antibiotic resistance among men who have sex with men: an observational study.
Whole-genome sequencing reveals transmission of gonococcal antibiotic resistance among men who have sex with men: an observational study.
复制标题
DOI：
10.1136/sextrans-2017-053287
复制
发表时间：
2018-03
影响因子：
3.6
通讯作者：
Howden BP
中科院分区：
文献类型：
--
作者：
Kwong JC;Chow EPF;Stevens K;Stinear TP;Seemann T;Fairley CK;Chen MY;Howden BP
耐药性淋病奈瑟菌现已成为全球公共卫生威胁。个体之间的直接传播的耐药性淋球菌已被提出作为一个驱动器的耐药性传播增加，但这种传播的直接证据是有限的。与传统的分子分型方法如多位点序列分型（MLST）和N。淋病多抗原序列（NG-MAST）。因此，我们的目的是系统地调查N。使用WGS在基因组水平上比较性伴侣中男性淋病的分离株及其对抗生素的耐药性。纳入了2005年至2014年期间一起进行淋病检测的男性与男性发生性关系（MSM）的大型前瞻性队列中的458对夫妇，并对来自两名男性均为N培养阳性的夫妇的所有分离株进行了WGS。淋病从基因组组装中鉴定耐药决定序列，并通过成对单核苷酸多态性和泛基因组比较以及NG-MAST和MLST的计算机预测来比较个体之间和个体内的分离株。对于34对夫妇中的33对（97%; 95%CI 85%至100%），其中双方均为淋病阳性，耐药决定基因和突变在来自每个伴侣的分离株中相同（共94株）。23例多部位感染男性中的23例（100%; 95%CI 86%至100%）分离株的耐药决定因素在个体内也相同。通过NG-MAST、MLST和全基因组比较，这些伴侣和宿主内分离株无法区分。这些数据支持性伴侣之间的耐药菌株传播是MSM淋病耐药率的关键驱动因素。这提高了对N.性伴侣之间的淋病将告知治疗和预防指南。
Drug-resistant Neisseria gonorrhoeae are now a global public health threat. Direct transmission of antibiotic-resistant gonococci between individuals has been proposed as a driver for the increased transmission of resistance, but direct evidence of such transmission is limited. Whole-genome sequencing (WGS) has superior resolution to investigate outbreaks and disease transmission compared with traditional molecular typing methods such as multilocus sequence typing (MLST) and N. gonorrhoeae multiantigen sequence (NG-MAST). We therefore aimed to systematically investigate the transmission of N. gonorrhoeae between men in sexual partnerships using WGS to compare isolates and their resistance to antibiotics at a genome level. 458 couples from a large prospective cohort of men who have sex with men (MSM) tested for gonorrhoea together between 2005 and 2014 were included, and WGS was conducted on all isolates from couples where both men were culture-positive for N. gonorrhoeae. Resistance-determining sequences were identified from genome assemblies, and comparison of isolates between and within individuals was performed by pairwise single nucleotide polymorphism and pangenome comparisons, and in silico predictions of NG-MAST and MLST. For 33 of 34 (97%; 95% CI 85% to 100%) couples where both partners were positive for gonorrhoea, the resistance-determining genes and mutations were identical in isolates from each partner (94 isolates in total). Resistance determinants in isolates from 23 of 23 (100%; 95% CI 86% to 100%) men with multisite infections were also identical within an individual. These partner and within-host isolates were indistinguishable by NG-MAST, MLST and whole genomic comparisons. These data support the transmission of antibiotic-resistant strains between sexual partners as a key driver of resistance rates in gonorrhoea among MSM. This improved understanding of the transmission dynamics of N. gonorrhoeae between sexual partners will inform treatment and prevention guidelines.
登录
查看更多内容
影响因子：
6.4
作者：
Didelot X;Dordel J;Whittles LK;Collins C;Bilek N;Bishop CJ;White PJ;Aanensen DM;Parkhill J;Bentley SD;Spratt BG;Harris SR
通讯作者：
Harris SR
影响因子：
56.3
作者：
Grad, Yonatan H.;Kirkcaldy, Robert D.;Trees, David;Dordel, Janina;Harris, Simon R.;Goldstein, Edward;Weinstock, Hillard;Parkhill, Julian;Hanage, William P.;Bentley, Stephen;Lipsitch, Marc
通讯作者：
Lipsitch, Marc
影响因子：
9.4
作者：
Demczuk, Walter;Martin, Irene;Mulvey, Michael R.
通讯作者：
Mulvey, Michael R.
影响因子：
9.4
作者：
Demczuk, Walter;Lynch, Tarah;Mulveya, Michael R.
通讯作者：
Mulveya, Michael R.
DOI：
10.1099/00221287-139-11-2603
发表时间：
1993-11-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
OROURKE, M;STEVENS, E
通讯作者：
STEVENS, E