Vagus Nerves Provide a Robust Afferent Innervation of the Mucosa Throughout the Body of the Esophagus in the Mouse.
Vagus Nerves Provide a Robust Afferent Innervation of the Mucosa Throughout the Body of the Esophagus in the Mouse.
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作者：
迷走传入神经调节吞咽和食管运动反射。然而，在食管粘膜迷走神经传入神经支配的认识上仍有差距。解剖学研究发现，迷走神经传入粘膜神经分布在上食管括约肌区是密集的，但在更远段的食管很少。与此相反，电生理学研究得出结论，迷走神经传入神经纤维也密集地支配更远端的食管粘膜。我们假设用编码绿色荧光蛋白的腺相关病毒载体（AAV-GFP）转染迷走神经传入神经元可以使小鼠食管粘膜中的迷走神经传入神经纤维可视化。将AAV-GFP注射到体内迷走神经颈静脉/结状神经节中以稀疏地标记迷走神经传入神经纤维。4-6周后收集食管组织，通过免疫染色放大GFP信号，并获得整个食管的共聚焦光学切片。我们在整个食管的粘膜中发现了大量GFP标记的纤维。GFP标记的粘膜纤维位于上皮下方，反复分支，大多数纵向取向，并突然终止而不形成终端结构。GFP标记的粘膜纤维集中在各种大小的随机区域，其中许多纤维可以追溯到一个单一的父母轴突。我们的结论是迷走神经提供了一个强大的传入神经支配的粘膜在整个身体的食道在小鼠。迷走粘膜纤维可能有助于感知管腔内内容物和调节吞咽和其他反射。
The vagal afferent nerves regulate swallowing and esophageal motor reflexes. However, there are still gaps in the understanding of vagal afferent innervation of the esophageal mucosa. Anatomical studies found that the vagal afferent mucosal innervation is dense in the upper esophageal sphincter area but rare in more distal segments of the esophagus. In contrast, electrophysiological studies concluded that the vagal afferent nerve fibers also densely innervate mucosa in more distal esophagus. We hypothesized that the transfection of vagal afferent neurons with adeno-associated virus vector encoding green fluorescent protein (AAV-GFP) allows to visualize vagal afferent nerve fibers in the esophageal mucosa in the mouse. AAV-GFP was injected into the vagal jugular/nodose ganglia in vivo to sparsely label vagal afferent nerve fibers. The esophageal tissue was harvested 4–6 weeks later, the GFP signal was amplified by immunostaining, and confocal optical sections of the entire esophagi were obtained. We found numerous GFP-labeled fibers in the mucosa throughout the whole body of the esophagus. The GFP-labeled mucosal fibers were located just beneath the epithelium, branched repeatedly, had mostly longitudinal orientation, and terminated abruptly without forming terminal structures. The GFP-labeled mucosal fibers were concentrated in random areas of various sizes in which many fibers could be traced to a single parental axon. We conclude that the vagus nerves provide a robust afferent innervation of the mucosa throughout the whole body of the esophagus in the mouse. Vagal mucosal fibers may contribute to the sensing of intraluminal content and regulation of swallowing and other reflexes.
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影响因子：
5.5
作者：
Kollarik, M.;Carr, M. J.;Undem, B. J.
通讯作者：
Undem, B. J.
影响因子：
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作者：
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通讯作者：
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影响因子：
2.7
作者：
Raab, M;Wörl, R;Neuhuber, WL
通讯作者：
Neuhuber, WL
影响因子：
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作者：
Kollarik, M;Dinh, QT;Undem, BJ
通讯作者：
Undem, BJ
DOI：
10.1152/ajpgi.00291.2016
发表时间：
2016-11-01
影响因子：
4.5
作者：
Lang, Ivan M.;Medda, Bidyut K.;Shaker, Reza
通讯作者：
Shaker, Reza