FluxNorm: Toolbox for metabolic flux assay normalization by in situ cell counting.
FluxNorm: Toolbox for metabolic flux assay normalization by in situ cell counting.
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FluxNorm:通过原位细胞计数进行代谢通量测定标准化的工具箱。
DOI:
10.1101/2023.10.13.562314
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发表时间:
2023
期刊:
影响因子:
--
通讯作者:
Pekkurnaz,Gulcin
中科院分区:
文献类型:
--
作者:
Djaja,NathalieA;Bracha,Teva;Yu,SeungyoonB;Wang,Haoming;Carlson,NatashaM;Pekkurnaz,Gulcin
Plate-based quantitative metabolic flux analysis has emerged as the central technology to examine cellular metabolism and mitochondrial bioenergetics. However, accurate interpretation of metabolic activity between different experimental conditions in multi-well microplates requires data normalization based on in situ cell counts. Here, we describe FluxNorm, a platform-independent semi-automated computational workflow, validated for three different cell types, to normalize cell density for accurate assessment of cellular bioenergetics.
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影响因子:
2.9
作者:
M. O'Leary;J. Hermes
通讯作者:
J. Hermes
DOI:
--
发表时间:
1966
期刊:
影响因子:
--
作者:
H. Adolph;M. J. Kamlet
通讯作者:
M. J. Kamlet
影响因子:
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作者:
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通讯作者:
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影响因子:
3.9
作者:
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通讯作者:
M. O'Leary
影响因子:
3.9
作者:
Hwang,SH;Nowak,T
通讯作者:
Nowak,T