Effects of Lactic Acid Bacterial Inoculants on Fermentation Quality, Bacterial Community, and Mycotoxins of Alfalfa Silage under Vacuum or Nonvacuum Treatment.
Effects of Lactic Acid Bacterial Inoculants on Fermentation Quality, Bacterial Community, and Mycotoxins of Alfalfa Silage under Vacuum or Nonvacuum Treatment.
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真空与非真空处理下乳酸菌剂对苜蓿青贮饲料发酵品质、细菌群落及霉菌毒素的影响
DOI：
10.3390/microorganisms9122614
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发表时间：
2021-12-17
期刊：
影响因子：
4.5
通讯作者：
Wang Y
中科院分区：
文献类型：
--
作者：
Fan X;Zhao S;Yang F;Wang Y;Wang Y
为了研究乳酸菌(LAB)接种剂和真空对紫花苜蓿发酵品质和细菌群落的影响，分别在真空(V)和非真空(NV)处理条件下，对紫花苜蓿进行了10天、30天、60天和90天的发酵试验。90d后，采用高通量测序法对紫花苜蓿青贮料中的微生物群落进行分析，并测定了青贮料中黄曲霉毒素B1和脱氧雪腐镰刀菌烯醇(DON)的含量。在所有接种的紫花苜蓿青贮料中，无论V或NV处理，乳酸(LA)含量增加，pH(p<；0.05)和氨氮(p<；0.05)含量降低，没有检测到丁酸。无论添加哪种菌剂，紫花苜蓿青贮料中的乳杆菌或链球菌属均以优势种为主，加希氏菌的丰度降低。室内接种菌剂YX和ZZUA493有助于降低紫花苜蓿青贮料中的真菌毒素含量。对照苜蓿青贮组的加希氏菌丰度和NV组的DON含量均高于V组(p<；0.05)。综上所述，LAB接种剂和真空处理对紫花苜蓿青贮品质有积极的影响，LAB接种剂能有效降低青贮料中的霉菌毒素。
To investigate the effects of lactic acid bacterial (LAB) inoculants and vacuuming on the fermentation quality and bacterial community, alfalfas were ensiled with or without a commercial LAB YX or Lactobacillus plantarum strain ZZUA493 for 10, 30, 60, and 90 days while undergoing either vacuum (V) or nonvacuum (NV) treatment. At 90 days, analysis of the microbial community by high-throughput sequencing was performed, and contents of aflatoxin B1 and deoxynivalenol (DON) mycotoxins in alfalfa silage were determined. In all inoculated alfalfa silage, irrespective of V or NV treatment, lactic acid (LA) content increased, pH (p < 0.05), and ammonia nitrogen (p < 0.05) content decreased, and no butyric acid was detected. Lactobacillus or Pediococcus became the dominant genus, and the abundance of Garciella decreased in alfalfa silage with the addition of either inoculant. The LAB inoculants YX and ZZUA493 helped reduce the mycotoxin content in alfalfa silage. The abundance of Garciella in the control and DON content in all alfalfa silage groups were higher (p < 0.05) in NV than V. In summary, LAB inoculants and vacuuming had a positive influence on alfalfa silage quality, and LAB inoculants were effective in reducing mycotoxins in silage alfalfa.
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影响因子：
2.4
作者：
Bruening, D.;Gerlach, K.;Suedekum, K. -H.
通讯作者：
Suedekum, K. -H.
接种重组本土和外源附生微生物群的红三叶草青贮饲料的微生物多样性和发酵概况
影响因子：
11.4
作者：
Ali, Niaz;Wang, Siran;Shao, Tao
通讯作者：
Shao, Tao
影响因子：
3.5
作者：
Ogunade, I. M.;Jiang, Y.;Adesogan, A. T.
通讯作者：
Adesogan, A. T.
影响因子：
3.2
作者：
Liu, Q. H.;Wu, J. X.;Shao, T.
通讯作者：
Shao, T.
影响因子：
3.5
作者：
Filya, I.;Muck, R. E.;Contreras-Govea, F. E.
通讯作者：
Contreras-Govea, F. E.