Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield.
Non-canonical regulation of SPL transcription factors by a human OTUB1-like deubiquitinase defines a new plant type rice associated with higher grain yield.
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人类 OTUB1 样去泛素酶对 SPL 转录因子的非规范调节定义了一种与更高谷物产量相关的新型水稻
DOI：
10.1038/cr.2017.98
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发表时间：
2017-09
期刊：
影响因子：
44.1
通讯作者：
Fu X
中科院分区：
文献类型：
--
作者：
Wang S;Wu K;Qian Q;Liu Q;Li Q;Pan Y;Ye Y;Liu X;Wang J;Zhang J;Li S;Wu Y;Fu X
实现谷物产量的提高一直是谷物育种计划的首要重点。理想型方法已在国际水稻研究所和中国用于提高水稻产量潜力。然而，水稻产量相关性状的遗传基础仍不清楚。在这里，我们表明，一个主要的数量性状基因座，qNPT 1，通过确定一个“新的植物类型”（NPT）的架构，其特征在于更少的分蘖，更坚固的秆和更大的圆锥花序的行为，它编码一个去泛素化酶与人类OTUB 1同源。OsOTUB 1的下调增强了水稻的分生组织活性，导致分蘖数减少，粒数增加，粒重增加，从而提高了水稻的产量。与人OTUB 1不同，OsOTUB 1可以切割K48和K63连接的多聚泛素。OsOTUB 1与E2泛素结合蛋白OsUBC 13和鳞状细胞启动子结合蛋白样转录因子OsSPL 14相互作用。OsOTUB 1和OsSPL 14具有共同的靶基因，它们的物理相互作用限制了OsSPL 14的K63连接的泛素化（K63 Ub），这反过来又促进了OsSPL 14的K48 Ub依赖的蛋白酶体降解。相反，OsOTUB 1的功能丧失与高水平OsSPL 14的积累相关，导致NPT结构。我们还证明了高产npt 1和dep 1 -1等位基因的重复使用为提高水稻产量潜力提供了一种新的策略。
Achieving increased grain productivity has long been the overriding focus of cereal breeding programs. The ideotype approach has been used to improve rice yield potential at the International Rice Research Institute and in China. However, the genetic basis of yield-related traits in rice remains unclear. Here, we show that a major quantitative trait locus, qNPT1, acts through the determination of a'new plant type'(NPT) architecture characterized by fewer tillers, sturdier culms and larger panicles, and it encodes a deubiquitinating enzyme with homology to human OTUB1. Downregulation of OsOTUB1 enhances meristematic activity, resulting in reduced tiller number, increased grain number, enhanced grain weight and a consequent increase in grain yield in rice. Unlike human OTUB1, OsOTUB1 can cleave both K48-and K63-linked polyubiquitin. OsOTUB1 interacts with the E2 ubiquitin-conjugating protein OsUBC13 and the squamosa promoter-binding protein-like transcription factor OsSPL14. OsOTUB1 and OsSPL14 share common target genes, and their physical interaction limits K63-linked ubiquitination (K63Ub) of OsSPL14, which in turn promotes K48Ub-dependent proteasomal degradation of OsSPL14. Conversely, loss-of-function of OsOTUB1 is correlated with the accumulation of high levels of OsSPL14, resulting in the NPT architecture. We also demonstrated that pyramiding of high-yielding npt1 and dep1-1 alleles provides a new strategy for increasing rice yield potential above what is currently achievable.
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影响因子：
5.6
作者：
Radjacommare R;Usharani R;Kuo CH;Fu H
通讯作者：
Fu H
影响因子：
27.5
作者：
Ma, Xingliang;Zhang, Qunyu;Liu, Yao-Guang
通讯作者：
Liu, Yao-Guang
DOI：
10.1073/pnas.132266399
发表时间：
2002-06-25
影响因子：
11.1
作者：
Spielmeyer, W;Ellis, MH;Chandler, PM
通讯作者：
Chandler, PM
为什么与 K63-多聚泛素链连接的细胞蛋白不与蛋白酶体结合？
影响因子：
11.4
作者：
Nathan, James A.;Kim, Hyoung Tae;Ting, Lily;Gygi, Steven P.;Goldberg, Alfred L.
通讯作者：
Goldberg, Alfred L.
OsSPL13 控制栽培稻的颗粒大小
影响因子：
30.8
作者：
Si, Lizhen;Chen, Jiaying;Han, Bin
通讯作者：
Han, Bin