Melatonin attenuates expression of cyclooxygenase-2 (COX-2) in activated microglia induced by lipopolysaccharide (LPS)
Melatonin attenuates expression of cyclooxygenase-2 (COX-2) in activated microglia induced by lipopolysaccharide (LPS)
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褪黑激素减弱脂多糖 (LPS) 诱导的活化小胶质细胞中环氧合酶 2 (COX-2) 的表达
DOI：
10.1080/15287394.2019.1615019
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发表时间：
2019-04
期刊：
影响因子：
--
通讯作者：
Cai Tongjian
中科院分区：
文献类型：
--
作者：
Yao Chunyan;Liu Xiaoling;Zhou Zhengyu;Xiang Ying;Yuan Shuai;Xie Weijia;Zhou Meiyu;Hu Zeyao;Li Yafei;Ji Ailing;Cai Tongjian
摘要 脂多糖（LPS）是一种已知的神经毒素，最广泛地用作小胶质细胞激活剂以诱导炎症性神经退行性变。褪黑素（MEL）是松果体的主要分泌产物，据报道其对……负责
ABSTRACT Lipopolysaccharide (LPS) is a known neurotoxin and utilized most extensively as a microglial activator for induction of inflammatory neurodegeneration. Melatonin (MEL) is the main secretory product of pineal gland reported to be responsible for a variety of physiological functions. However, the molecular mechanisms underlying the influence of MEL on microglia activation remain unclear. The aim of this study was to investigate the effect of MEL on cyclooxygenase-2 (COX-2) levels in LPS-induced microglia. The results of RT-PCR and Western blot analysis showed that MEL significantly inhibited LPS-mediated upregulation of COX-2 in microglia. Data from ELISA demonstrated that prostaglandin E2 (PGE2), the downstream effector of COX-2, concentrations were also reduced. In addition, MEL was found to decrease activation of ERK1/2, JNK, p38 MAPK, and NF-κB, the upstream signal pathways of COX-2. Taken together, evidence indicates that MEL may attenuate upregulation of COX-2 by blocking the MAPK/NF-κB signaling pathway in LPS-stimulated microglia.
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DOI：
10.1080/15287394.2017.1384171
发表时间：
2017-10
期刊：
Journal of Toxicology and Environmental Health Part A: Current Issues
影响因子：
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作者：
Shao Guangfang;Zhang Suping;Nie Jihua;Li Jianxiang;Tong Jian
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Shiow LR;Favrais G;Schirmer L;Schang AL;Cipriani S;Andres C;Wright JN;Nobuta H;Fleiss B;Gressens P;Rowitch DH
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Aparicio-Soto, M.;Alarcon-de-la-Lastra, C.;Sanchez-Hidalgo, M.
通讯作者：
Sanchez-Hidalgo, M.
影响因子：
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Land, Tiit