Nucleolin/Nsr1p binds to the 3' noncoding region of the tombusvirus RNA and inhibits replication.
Nucleolin/Nsr1p binds to the 3' noncoding region of the tombusvirus RNA and inhibits replication.
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DOI：
10.1016/j.virol.2009.10.007
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发表时间：
2010-01-05
期刊：
影响因子：
3.7
通讯作者：
Nagy, Peter D.
中科院分区：
文献类型：
--
作者：
Jiang, Yi;Li, Zhenghe;Nagy, Peter D.
先前的全基因组筛选使用酵母模型宿主鉴定了>100个影响番茄丛矮病毒复制的宿主基因。其中一个因子是Nsr 1 p（核仁素），它是一种丰富的RNA结合穿梭蛋白，参与rRNA成熟和核糖体组装。我们发现，在酵母或本氏烟草中过表达Nsr 1 p可抑制番茄丛矮病毒RNA的积累约10倍。Nsr 1 p的过度表达表明，Nsr 1 p应存在于病毒复制的开始有效抑制，这表明Nsr 1 p抑制复制过程中的早期步骤。体外实验显示Nsr 1 p优选结合病毒RNA中的3' UTR。纯化的重组Nsr 1 p抑制在体外复制的病毒RNA在酵母细胞无试验中，当预孵育的病毒RNA前的测定。这些数据支持Nsr 1 p/核仁素通过干扰病毒RNA的募集来抑制Tombusvirus复制的模型。
Previous genome-wide screens identified >100 host genes affecting tombusvirus replication using yeast model host. One of those factors was Nsr1p (nucleolin), which is an abundant RNA binding shuttle protein involved in rRNA maturation and ribosome assembly. We find that over-expression of Nsr1p in yeast or in Nicotiana benthamiana inhibited the accumulation of tombusvirus RNA by ~10-fold. Regulated over-expression of Nsr1p revealed that Nsr1p should be present at the beginning of viral replication for efficient inhibition, suggesting that Nsr1p inhibits an early step in the replication process. In vitro experiments revealed that Nsr1p binds preferably to the 3' UTR in the viral RNA. The purified recombinant Nsr1p inhibited the in vitro replication of the viral RNA in a yeast cell-free assay when pre-incubated with the viral RNA before the assay. These data support the model that Nsr1p/nucleolin inhibits tombusvirus replication by interfering with the recruitment of the viral RNA for replication.
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影响因子：
3.7
作者：
Li, Zhenghe;Pogany, Judit;Panavas, Tadas;Xu, Kai;Esposito, Anthony M.;Kinzy, Terri Goss;Nagy, Peter D.
通讯作者：
Nagy, Peter D.
影响因子：
3.7
作者：
Nagy, PD;Pogany, J
通讯作者：
Pogany, J
DOI：
10.1016/j.bbrc.2007.10.117
发表时间：
2008-01-04
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
64.8
作者：
Krishnan, Manoj N.;Ng, Aylwin;Sukumaran, Bindu;Gilfoy, Felicia D.;Uchil, Pradeep D.;Sultana, Hameeda;Brass, Abraham L.;Adametz, Rachel;Tsui, Melody;Qian, Feng;Montgomery, Ruth R.;Lev, Sima;Mason, Peter W.;Koski, Raymond A.;Elledge, Stephen J.;Xavier, Ramnik J.;Agaisse, Herve;Fikrig, Erol
通讯作者：
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