Possible role of DMP1 in dentin mineralization.
Possible role of DMP1 in dentin mineralization.
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DOI：
10.1016/j.jsb.2010.11.013
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发表时间：
2011-04
影响因子：
3
通讯作者：
Sfeir, Charles S.
中科院分区：
文献类型：
--
作者：
Beniash, Elia;Deshpande, Atul S.;Fang, Ping An;Lieb, Nicholas S.;Zhang, Xiaoyuan;Sfeir, Charles S.
牙本质基质蛋白1(DMP1)是牙本质和骨组织中必不可少的非胶原蛋白，在这些组织的矿化过程中发挥着重要作用，但其作用机制尚不完全清楚。为了深入了解DMP1在牙本质矿化中的作用，我们在完全矿化的大鼠切牙上进行了DMP1的免疫映射，并在存在DMP1的情况下进行了体外钙磷矿化实验。DMP1免疫荧光定位于小管周牙本质和牙本质-牙釉质交界处。在体外，磷酸化的DMP1诱导形成c轴共同排列的平行微晶阵列。这种晶体排列是骨和牙本质矿化的胶原纤维的标志。有趣的是，在富含DMP1的PTD中，没有胶原纤维，晶体以类似的方式组织。根据我们的发现，我们推测，在体内，DMP1控制着胶原纤维外的矿物质组织，并在PTD的矿化过程中发挥主要作用。
Dentin Matrix Protein 1 (DMP1), the essential noncollagenous proteins in dentin and bone, is believed to play an important role in the mineralization of these tissues, although the mechanisms of its action are not fully understood. To gain insight into DMP1 functions in dentin mineralization we have performed immunomaping of DMP1 in fully mineralized rat incisors and in vitro calcium phosphate mineralization experiments in the presence of DMP1. DMP1 immunofluorescene was localized in peritubular dentin (PTD) and along the dentin-enamel boundary. In vitro phosphorylated DMP1 induced the formation of parallel arrays of crystallites with their c-axes co-aligned. Such crystalline arrangement is a hallmark of mineralized collagen fibrils of bone and dentin. Interestingly, in DMP1-rich PTD, which lacks collagen fibrils, the crystals are organized in a similar manner. Based on our findings we hypothesize, that in vivo DMP1 controls the mineral organization outside of the collagen fibrils and plays a major role in the mineralization of PTD.
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影响因子：
5.1
作者：
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影响因子：
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