Sequence and annotation of the apicoplast genome of the human pathogen Babesia microti.
Sequence and annotation of the apicoplast genome of the human pathogen Babesia microti.
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DOI：
10.1371/journal.pone.0107939
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ben Mamoun C
中科院分区：
文献类型：
--
作者：
Garg A;Stein A;Zhao W;Dwivedi A;Frutos R;Cornillot E;Ben Mamoun C
顶复合体红细胞内寄生虫微小巴贝斯虫是一种新出现的人类病原体，也是人类巴贝斯虫病的主要原因，巴贝斯病是一种在美国流行的类似疟疾的疾病。这种病原体通过扁虱媒介肩部硬蜱传播给人类，并通过输血传播给无症状的微小硬蜱感染捐赠者。虽然该寄生虫的核基因组和线粒体基因组已被测序、组装和注释，但其质外体基因组仍然不完整，这主要是由于其低代表性和高A+T含量。在这里，我们报道了B.microti R1分离物的质外体基因组的完整序列和注释。基因组由28.7kb的环状分子组成，编码对维持质外体DNA、细胞器蛋白质的转录、翻译和成熟至关重要的功能。基因组分析和注释揭示了B.microti质外体基因组独特的基因结构和组织，并表明该细胞器中的所有代谢和非内务功能都是核编码的。B.microti的质外体功能与宿主的显著不同，这表明它们可能是开发有效和安全的治疗人类巴贝斯虫病的靶标。
The apicomplexan intraerythrocytic parasite Babesia microti is an emerging human pathogen and the primary cause of human babesiosis, a malaria-like illness endemic in the United States. The pathogen is transmitted to humans by the tick vector, Ixodes scapularis, and by transfusion of blood from asymptomatic B. microti-infected donors. Whereas the nuclear and mitochondrial genomes of this parasite have been sequenced, assembled and annotated, its apicoplast genome remained incomplete, mainly due to its low representation and high A+T content. Here we report the complete sequence and annotation of the apicoplast genome of the B. microti R1 isolate. The genome consists of a 28.7 kb circular molecule encoding primarily functions important for maintenance of the apicoplast DNA, transcription, translation and maturation of organellar proteins. Genome analysis and annotation revealed a unique gene structure and organization of the B. microti apicoplast genome and suggest that all metabolic and non-housekeeping functions in this organelle are nuclear-encoded. B. microti apicoplast functions are significantly different from those of the host, suggesting that they might be useful as targets for development of potent and safe therapies for the treatment of human babesiosis.
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影响因子：
158.5
作者：
Krause, PJ;Spielman, A;Persing, DH
通讯作者：
Persing, DH
影响因子：
4.4
作者：
Hikosaka，Kenji;Tsuji，Naotoshi;Tanabe，Kazuyuki
通讯作者：
Tanabe，Kazuyuki
影响因子：
14.9
作者：
Dereeper A;Guignon V;Blanc G;Audic S;Buffet S;Chevenet F;Dufayard JF;Guindon S;Lefort V;Lescot M;Claverie JM;Gascuel O
通讯作者：
Gascuel O
影响因子：
14.9
作者：
Finn RD;Mistry J;Tate J;Coggill P;Heger A;Pollington JE;Gavin OL;Gunasekaran P;Ceric G;Forslund K;Holm L;Sonnhammer EL;Eddy SR;Bateman A
通讯作者：
Bateman A
影响因子：
4.4
作者：
Vannier E;Gewurz BE;Krause PJ
通讯作者：
Krause PJ