Non-condensing polymeric nanoparticles for targeted gene and siRNA delivery.
Non-condensing polymeric nanoparticles for targeted gene and siRNA delivery.
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DOI：
10.1016/j.ijpharm.2011.05.036
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发表时间：
2012-05-01
影响因子：
5.8
通讯作者：
Amiji, Mansoor
中科院分区：
文献类型：
--
作者：
Xu, Jing;Ganesh, Shanthi;Amiji, Mansoor
关键词：
基因治疗已经显示出巨大的潜力，使患者在各种疾病条件下受益。然而，寻找一种安全有效的全身给药系统是这一领域的主要障碍。尽管病毒载体显示出高转染率的前景，但与这些系统相关的免疫原性阻碍了进一步的发展。作为一种替代病毒基因传递，这篇评论的重点是应用新的安全和有效的非冷凝聚合物系统，系统或口服给药时，表现出高的转基因表达。使用报告基因和治疗性质粒DNA评估基于B型明胶的工程化纳米载体的被动和主动肿瘤靶向递送和转染。此外，我们已经表明，纳米微球口服系统（NiMOS）可以有效地提供报告和治疗基因构建体在胃肠道。此外，最近对使用小干扰RNA（siRNA）作为基因沉默的治疗系统有很大的兴趣。明胶纳米颗粒和NiMOS都分别在siRNA的全身和口服递送中显示出活性。
Gene therapy has shown a tremendous potential to benefit patients in a variety of disease conditions. However, finding a safe and effective systemic delivery system is the major obstacle in this area. Although viral vectors showed promise for high transfection rate, the immunogenicity associated with these systems has hindered further development. As an alternative to viral gene delivery, this review focuses on application of novel safe and effective non-condensing polymeric systems that have shown high transgene expression when administered systemically or by the oral route. Type B gelatin-based engineered nanocarriers were evaluated for passive and active tumor-targeted delivery and transfection using both reporter and therapeutic plasmid DNA. Additionally, we have shown that nanoparticles-in-microsphere oral system (NiMOS) can efficiently deliver reporter and therapeutic gene constructs in the gastrointestinal tract. Additionally, there has been a significant recent interest in the use small interfering RNA (siRNA) as a therapeutic system for gene silencing. Both gelatin nanoparticles and NiMOS have shown activity in systemic and oral delivery of siRNA, respectively.
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影响因子：
5.1
作者：
Bhavsar, M. D.;Amiji, M. M.
通讯作者：
Amiji, M. M.
影响因子：
10.8
作者：
Bhavsar, MD;Tiwari, SB;Amiji, MM
通讯作者：
Amiji, MM
影响因子：
64.8
作者：
Hoag, Hannah
通讯作者：
Hoag, Hannah
DOI：
10.1038/nrd2310
发表时间：
2007-06
期刊：
Nature reviews. Drug discovery
影响因子：
--
作者：
de Fougerolles A;Vornlocher HP;Maraganore J;Lieberman J
通讯作者：
Lieberman J
影响因子：
10.8
作者：
Bhavsar, Mayank D.;Amiji, Mansoor M.
通讯作者：
Amiji, Mansoor M.