The adult Drosophila testis lacks a mechanism to replenish missing niche cells.
The adult Drosophila testis lacks a mechanism to replenish missing niche cells.
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DOI：
10.1242/dev.201148
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发表时间：
2023-01-15
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成年果蝇睾丸包含一个由一群中枢细胞创建的明确的小生境，这些中枢细胞分泌维持相邻生殖系干细胞和体细胞囊肿干细胞（CySC）的信号。枢纽细胞在成年果蝇中通常是静止的，但在所有CySC消融后可以退出静止，从枢纽分层并转化为CySC。相反的事件，CySC转化为枢纽细胞，被认为是在生理条件下发生的，但这种事件的频率是有争议的。在这里，为了进一步探索枢纽细胞是否可以再生的问题，我们开发了基因消融部分或全部枢纽细胞的方法。令人惊讶的是，当苍蝇被允许从消融中恢复时，缺失的枢纽细胞没有被替换。在部分消融轮毂细胞后，轮毂细胞未退出静止期，消融期间或消融后，来自轮毂外部的标记细胞未进入轮毂。尽管它的能力，退出静止响应CySC消融，我们得出结论，在成年果蝇睾丸枢纽没有一个机制，以补充缺失的枢纽细胞。 成年果蝇睾丸中的非分裂小生境细胞，能够分裂以取代缺失的干细胞，但不能取代自己。
The adult Drosophila testis contains a well-defined niche created by a cluster of hub cells, which secrete signals that maintain adjacent germline stem cells and somatic cyst stem cells (CySCs). Hub cells are normally quiescent in adult flies but can exit quiescence, delaminate from the hub and convert into CySCs after ablation of all CySCs. The opposite event, CySC conversion into hub cells, was proposed to occur under physiological conditions, but the frequency of this event is debated. Here, to probe further the question of whether or not hub cells can be regenerated, we developed methods to genetically ablate some or all hub cells. Surprisingly, when flies were allowed to recover from ablation, the missing hub cells were not replaced. Hub cells did not exit quiescence after partial ablation of hub cells, and labeled cells from outside the hub did not enter the hub during or after ablation. Despite its ability to exit quiescence in response to CySC ablation, we conclude that the hub in the adult Drosophila testis does not have a mechanism to replenish missing hub cells. Non-dividing niche cells in the adult Drosophila testis, which are able to divide to replace missing stem cells, are unable to replace themselves.
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