Transcriptome analysis reveals the genetic basis underlying the biosynthesis of volatile oil, gingerols, and diarylheptanoids in ginger (Zingiber officinale Rosc.).
Transcriptome analysis reveals the genetic basis underlying the biosynthesis of volatile oil, gingerols, and diarylheptanoids in ginger (Zingiber officinale Rosc.).
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转录组分析揭示了生姜（Zingiber officinale Rosc.）中挥发油、姜辣素和二芳基庚烷类生物合成的遗传基础
DOI：
10.1186/s40529-017-0195-5
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发表时间：
2017-10-23
影响因子：
3.4
通讯作者：
Lan J
中科院分区：
文献类型：
--
作者：
Jiang Y;Liao Q;Zou Y;Liu Y;Lan J
背景：生姜（Ginger（Escherber officinale Rosc.）生姜是一种在亚洲广泛使用的流行调味品，生姜根茎中的挥发油为食物增添了特殊的香味和味道。生姜中的生物活性化合物，如姜辣素，二芳基庚烷类化合物和黄酮类化合物，由于其抗癌，抗氧化和抗炎特性，对人类健康具有重要价值。然而，作为一种非模式植物，有关生姜基因组序列的知识非常有限，这限制了对这种植物的分子研究。本研究采用转录组从头测序技术，对生姜成熟根茎（MG）、生姜幼根（YG）和生姜须根（FR）中主要生物活性物质生物合成相关基因的表达进行了研究。参与主要生物活性化合物生物合成途径的基因表达在组织（MG、YG和FR）之间存在差异。产生挥发油、姜辣素和二芳基庚烷类化合物的两种途径，“萜类化合物骨架生物合成”和“芪类化合物、二芳基庚烷类化合物和姜辣素生物合成”途径，在FR和YG文库以及FR和MG文库之间的差异表达基因（DEG）（FDR < 0.005）中显著富集（调整的P值< 0.05）。在这两条途径中定位的大多数单基因，包括姜黄素合成酶、苯丙酰乙酰辅酶A合成酶、反式肉桂酸4-单加氧酶和4-羟基-3-甲基丁-2-烯-1-基二磷酸合成酶，表达水平显著较高（log 2（倍数变化）≥ 1）。结论：本研究首次从分子水平上揭示了生姜中生物活性物质的生物合成，为生姜的分子生物学研究提供了宝贵的基因组资源。研究生姜。此外，我们的研究结果表明，生姜中的生物活性化合物可能主要在根部合成，然后运输到根茎，在那里它们积累。
Background:Ginger (Zingiber officinale Rosc.) is a popular flavoring that widely used in Asian, and the volatile oil in ginger rhizomes adds a special fragrance and taste to foods. The bioactive compounds in ginger, such as gingerols, diarylheptanoids, and flavonoids, are of significant value to human health because of their anticancer, anti-oxidant, and anti-inflammatory properties. However, as a non-model plant, knowledge about the genome sequences of ginger is extremely limited, and this limits molecular studies on this plant. In this study, de novo transcriptome sequencing was performed to investigate the expression of genes associated with the biosynthesis of major bioactive compounds in matured ginger rhizome (MG), young ginger rhizome (YG), and fibrous roots of ginger (FR).Results:A total of 361,876 unigenes were generated by de novo assembly. The expression of genes involved in the pathways responsible for the biosynthesis of major bioactive compounds differed between tissues (MG, YG, and FR). Two pathways that give rise to volatile oil, gingerols, and diarylheptanoids, the "terpenoid backbone biosynthesis" and "stilbenoid, diarylheptanoid and gingerol biosynthesis" pathways, were significantly enriched (adjusted P value < 0.05) for differentially expressed genes (DEGs) (FDR < 0.005) both between the FR and YG libraries, and the FR and MG libraries. Most of the unigenes mapped in these two pathways, including curcumin synthase, phenylpropanoylacetyl-CoA synthase, trans-cinnamate 4-monooxygenase, and 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, were expressed to a significantly higher level (log2(fold-change) ≥ 1) in FR than in YG or MG.Conclusion:This study provides the first insight into the biosynthesis of bioactive compounds in ginger at a molecular level and provides valuable genome resources for future molecular studies on ginger. Moreover, our results establish that bioactive compounds in ginger may predominantly synthesized in the root and then transported to rhizomes, where they accumulate.
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影响因子：
5.6
作者：
Ghasemzadeh A;Jaafar HZ;Rahmat A
通讯作者：
Rahmat A
DOI：
10.3390/molecules15096231
发表时间：
2010-09-03
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Ghasemzadeh A;Jaafar HZ;Rahmat A
通讯作者：
Rahmat A
来自甘蓝型油菜和亲本物种的透明测试 12 基因：克隆、进化和黄色种子性状的差异参与
影响因子：
3.1
作者：
Chai, You-Rong;Lei, Bo;Chen, Li
通讯作者：
Chen, Li
影响因子：
3.8
作者：
Ramirez-Ahumada, Maria Del Carmen;Timmermann, Barbara N.;Gang, David R.
通讯作者：
Gang, David R.
影响因子：
6.1
作者：
Sekiwa, Y;Kubota, K;Kobayashi, A
通讯作者：
Kobayashi, A