Effects of embryonic exposure to polychlorinated biphenyls on zebrafish (Danio rerio) retinal development
Effects of embryonic exposure to polychlorinated biphenyls on zebrafish (Danio rerio) retinal development
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胚胎暴露于多氯联苯对斑马鱼（Danio rerio）视网膜发育的影响
DOI：
10.1002/jat.1650
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发表时间：
2012-03
期刊：
影响因子：
--
通讯作者：
Yan-Ping Wang
中科院分区：
文献类型：
--
作者：
Qin Hong;*Xia Chia;Chun-Zhao Kou;Mei Guo;Da-ni Qin;Chun-Mei Zhang;Xi-Rong Guo;Mei-Ling Tong;Yan-Ping Wang
多氯联苯是影响胚胎发育的持久性环境污染物。本研究的目的是研究胚胎暴露于多氯联苯对斑马鱼早期视网膜发育的影响。将斑马鱼胚胎立即暴露于每升培养基中不同浓度（0、0.125、0.25、0.5、1.0和2.0 mg）的多氯联苯中，温度为28.5℃。在受精后30,48,72和96 h评估胚胎的胚胎存活率，发育，幼虫视网膜形态和视网膜超微结构的变化。结果表明，多氯联苯暴露降低胚胎存活率呈时间和剂量依赖性。胚胎暴露于较高浓度的多氯联苯（0.5、1.0和2.0 mg l - 1）时，表现出明显的大体形态畸形。在72 hpf时，较高的PCB浓度（1.0 mg l−1）会延迟斑马鱼视网膜层的发育。在96 hpf下，0.25-1 mg l - 1浓度的PCB处理的幼虫光敏细胞排列不规则，光敏细胞和神经节层增厚。超微结构检查显示，在72 hpf下暴露于0.25-1 mg l−1 PCB时，光感受器外节的生长受到抑制，并且在96 hpf下PCB暴露组的外节内出现大量液泡和孔洞。这些结果表明，胚胎暴露于中等和高水平的多氯联苯会导致斑马鱼视网膜的发育缺陷，尤其是感光细胞。版权所有©2011 John Wiley & Sons， Ltd
Polychlorinated biphenyls (PCBs) are persistent environmental pollutants that affect embryonic development. The purpose of this study was to examine the effects of embryonic exposure to PCBs on early retinal development in zebrafish, Danio rerio. Zebrafish embryos were immediately exposed to different concentrations (0, 0.125, 0.25, 0.5, 1.0 and 2.0 mg) of PCBs per liter of medium at 28.5 °C. Embryos were assessed at 30, 48, 72 and 96 h post‐fertilization (hpf) for changes in embryonic survival rate, development, larval retinal morphology and ultrastructure of the retina. The results show that PCB exposure decreased the survival rate of embryos in a time‐ and dose‐dependent manner. Embryos exposed to the higher concentrations of PCBs (0.5, 1.0 and 2.0 mg l−1) displayed obvious gross morphological deformities. At 72 hpf, the retinal layer development of zebrafish was delayed at higher PCB concentrations (1.0 mg l−1). At 96 hpf, irregularity of photoreceptor cells arrangement and thickening of photoreceptor and ganglionic layers were observed in PCB‐treated larvae at concentrations of 0.25–1 mg l−1. Ultrastructural examination showed signs of growth inhibition of the photoreceptor outer segment at 0.25–1 mg l−1 PCB exposure at 72 hpf, as well as the appearance of massive vacuoles and holes inside the outer segments in the PCB exposure group at 96 hpf. These results suggest that embryonic exposure to moderate and high levels of PCBs induced developmental deficits in zebrafish retinas, particularly in photoreceptor cells. Copyright © 2011 John Wiley & Sons, Ltd.
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影响因子：
3.4
作者：
H. Tilson;Kodavanti Pr
通讯作者：
H. Tilson;Kodavanti Pr
影响因子：
2.5
作者：
Hj. Müller
通讯作者：
Hj. Müller
DOI：
10.1016/b978-0-323-53094-1.00006-0
发表时间：
2019
期刊：
Perinatal Genetics
影响因子：
--
作者：
Stephanie H. Guseh;Rebecca Reimers;Louise Wilkins-Haug
通讯作者：
Stephanie H. Guseh;Rebecca Reimers;Louise Wilkins-Haug
影响因子：
3.3
作者：
K. Billsson;L. Westerlund;M. Tysklind;P. Olsson
通讯作者：
K. Billsson;L. Westerlund;M. Tysklind;P. Olsson
影响因子：
4.4
作者：
H. Bedell
通讯作者：
H. Bedell