Electrospray mass spectrometric characterization of fluoroquinolone antibiotics: Norfloxacin, enoxacin, ciprofloxacin and ofloxacin
Electrospray mass spectrometric characterization of fluoroquinolone antibiotics: Norfloxacin, enoxacin, ciprofloxacin and ofloxacin
复制标题
氟喹诺酮类抗生素：诺氟沙星、依诺沙星、环丙沙星和氧氟沙星的电喷雾质谱表征
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
L. Provost
中科院分区：
文献类型：
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作者：
P. D'agostino;J. Hancock;L. Provost
我们报道了四种氟喹诺酮类药物，依诺沙星，诺氟沙星，氧氟沙星和环丙沙星的电喷雾质谱，这是一组以前没有用这种电离技术表征的高效抗生素。在低采样锥电压设置（50和100 V）下，电喷雾质谱主要含有分子加合物离子。在150 V的采样锥电压下，产生了含有结构显著产物离子的碰撞激活解离质谱，并在3000的磁扇区分辨率下精确地测量了质量。利用填充毛细管柱液相色谱、紫外和ESI-MS检测，证实了这些化合物的梯度分离和表征。在电喷雾质谱分析中，估计了1 ~ 2ng范围内的紫外检测限（（信噪比S/N） = 10:1），并观察到1.5 ng分析物的可解释质谱的信噪比超过100:1。
We report the electrospray (ESI) mass spectra of four fluoroquinolones, enoxacin, norfloxacin, ofloxacin and ciprofloxacin, a group of highly potent antibiotics that have not been previously characterized by this ionization technique. At low sampling cone voltage settings, 50 and 100 V, the electrospray mass spectra contained primarily molecular adduct ions. At a sampling cone voltage setting of 150 V, collisionally activated dissociation mass spectra containing structurally significant product ions were produced and accurately mass measured at a magnetic sector resolution of 3000. Gradient separation and characterization of these compounds was demonstrated by analysis using packed capillary column liquid chromatography with UV and ESI-MS detection. UV detection limits in the 1 to 2 ng range ((signal-to-noise ratio S/N) = 10:1) were estimated and interpretable mass spectra with S/N ratios in excess of 100:1 were observed for 1.5 ng of analyte during electrospray mass spectrometric analysis.
4-喹诺酮抗生素的类选择性碰撞激活解离/离子分子反应。
影响因子：
7.4
作者：
Colorado,A;Brodbelt,J
通讯作者：
Brodbelt,J