The rhizobacterium Variovorax paradoxus 5C-2, containing ACC deaminase, promotes growth and development of Arabidopsis thaliana via an ethylene-dependent pathway.
The rhizobacterium Variovorax paradoxus 5C-2, containing ACC deaminase, promotes growth and development of Arabidopsis thaliana via an ethylene-dependent pathway.
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DOI：
10.1093/jxb/ert031
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发表时间：
2013-04
影响因子：
6.9
通讯作者：
Davies WJ
中科院分区：
文献类型：
--
作者：
Chen L;Dodd IC;Theobald JC;Belimov AA;Davies WJ
许多与植物根相关的植物促生根际细菌（PGPR）含有1-氨基环丙烷-1-羧酸（ACC）脱氨酶，并且可以代谢ACC（植物激素乙烯的直接前体），从而减少植物乙烯产生并增加植物生长。然而，相对较少的研究明确地将乙烯排放和/或作用与这些植物-微生物相互作用中的生长促进联系起来。本研究使用野生型（WT）植物和几种乙烯相关突变体（etr 1 -1、ein 2 -1和eto 1 -1）研究了含有ACC脱氨酶的PGPR Variovorax paradoxus 5C-2对拟南芥生长和发育的影响。土壤接种V. paradoxus 5C-2促进WT植物和乙烯过量产生突变体eto 1 -1的生长（叶面积和地上部生物量），并且还使WT植物的花起始增强2.5天。然而，这些影响没有看到乙烯不敏感的突变体（etr 1 -1和ein 2 -1），即使细菌定植的根系统是相似的。此外，V. paradoxus 5C-2使WT植物莲座叶的ACC浓度降低了59%，使WT植物和eto 1 -1突变体的叶片乙烯释放量分别降低了42%和37%。综上所述，这些结果表明，V. paradoxus 5C-2需要一个功能齐全的乙烯信号转导途径来刺激叶片生长和开花。thaliana.
Many plant-growth-promoting rhizobacteria (PGPR) associated with plant roots contain the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase and can metabolize ACC, the immediate precursor of the plant hormone ethylene, thereby decreasing plant ethylene production and increasing plant growth. However, relatively few studies have explicitly linked ethylene emission and/or action to growth promotion in these plant–microbe interactions. This study examined effects of the PGPR Variovorax paradoxus 5C-2 containing ACC deaminase on the growth and development of Arabidopsis thaliana using wild-type (WT) plants and several ethylene-related mutants (etr1-1, ein2-1, and eto1-1). Soil inoculation with V. paradoxus 5C-2 promoted growth (leaf area and shoot biomass) of WT plants and the ethylene-overproducing mutant eto1-1, and also enhanced floral initiation of WT plants by 2.5 days. However, these effects were not seen in ethylene-insensitive mutants (etr1-1 and ein2-1) even though bacterial colonization of the root system was similar. Furthermore, V. paradoxus 5C-2 decreased ACC concentrations of rosette leaves of WT plants by 59% and foliar ethylene emission of both WT plants and eto1-1 mutants by 42 and 37%, respectively. Taken together, these results demonstrate that a fully functional ethylene signal transduction pathway is required for V. paradoxus 5C-2 to stimulate leaf growth and flowering of A. thaliana.
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影响因子：
1.8
作者：
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作者：
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