Test of a novel Streptococcus pneumoniae serotype 6C type specific polyclonal antiserum (factor antiserum 6d) and characterisation of serotype 6C isolates in Denmark.
Test of a novel Streptococcus pneumoniae serotype 6C type specific polyclonal antiserum (factor antiserum 6d) and characterisation of serotype 6C isolates in Denmark.
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DOI：
10.1186/1471-2334-10-282
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发表时间：
2010-09-24
影响因子：
3.7
通讯作者：
Kerrn MB
中科院分区：
文献类型：
--
作者：
Lambertsen L;Kerrn MB
2007年，Park等人在肺炎链球菌血清组6中鉴定了一种新的血清型，他们将其命名为血清型6C。本研究的目的是用Neufeld试验评价一种新的S.肺炎血清型6C型特异性多克隆抗血清。此外，对2007年和2008年在丹麦发现的血清型6C分离株以及8株旧的原始血清型6A分离株进行了表征。在本研究中，检查了2007年和2008年来自丹麦的181株肺炎链球菌临床分离株; 96株分离株先前被分型为血清型6A，85株为血清型6 B。此外，从1952年至1987年，较早的血清型为6A的8个较老的分离株进行了检查。通过PCR和使用新型特异性多克隆抗血清、因子抗血清6 d以及因子抗血清6 b、6 b *（与血清型6C交叉反应的游离吸收）和6c的Neufeld试验进行血清分型来鉴定血清型6C分离株。所有抗血清均可商购获得，2009年1月1日后从供应商处获得的抗血清6 b为抗血清6 b *。使用多位点序列分型进一步表征所有血清型6C分离株。当通过PCR和Neufeld试验重新检测所有96株原始血清型6A分离株时，2007年和2008年丹麦29.6%（24/81）的侵袭性分离株被识别为血清型6C。此外，最初血清分型为6A的8个旧分离株中有3个被鉴定为血清型6C。最早的血清型6C分离株来自1962年。血清型6C分离株分属11种不同的序列型（ST）和9种克隆复合体（CC），以ST 1692（CC 395）、ST 386（CC 386）和ST 481（CC 460）为主。本研究制备了一种新的多克隆抗血清6d和经修饰的抗血清6 b *，为S.使用Neufeld试验对肺炎血清组6进行血清分型，并将血清分型方法与基于PCR的方法进行比较。这两种方法提供了相同的结果。因此，将来也有可能根据标准的S血清分型方法检测血清型6C。肺炎是WHO推荐的。在2007年和2008年来自丹麦的所有侵袭性分离株中，血清型6C占原始血清型6A分离株的29.6%。发现血清型6C分离株具有多样性，属于许多不同的ST和CC，其中大多数先前已在其他国家观察到。血清型6C被认为是存在于沙门氏菌中的“老”血清型。肺炎分离株在丹麦至少48年。血清型6C分离株的遗传多样性及其与其他血清型的遗传关系表明，血清型6C菌株可能是由涉及不同亲本菌株（如血清型6A、6 B、23 F和4）的几种不同的独立重组事件引起的。
In 2007, Park et al. identified a novel serotype among Streptococcus pneumoniae serogroup 6 which they named serotype 6C. The aim of this study was to evaluate with the Neufeld test a novel S. pneumoniae serotype 6C type specific polyclonal antiserum. In addition, serotype 6C isolates found in Denmark in 2007 and 2008 as well as eight old original serotype 6A isolates were characterised. In this study, 181 clinical Streptococcus pneumoniae isolates from Denmark 2007 and 2008 were examined; 96 isolates had previously been typed as serotype 6A and 85 as serotype 6B. In addition, eight older isolates from 1952 to 1987, earlier serotyped as 6A, were examined. Serotype 6C isolates were identified by PCR and serotyping with the Neufeld test using the novel type specific polyclonal antiserum, factor antiserum 6 d, in addition to factor antisera 6b, 6b* (absorbed free for cross-reactions to serotype 6C) and 6c. All antisera are commercially available and antiserum 6b obtained from the supplier after 1 January 2009 is antiserum 6b*. All serotype 6C isolates were further characterised using multi-locus sequence typing. When retesting all 96 original serotype 6A isolates by PCR and the Neufeld test, 29.6% (24 of 81) of the invasive isolates in Denmark from 2007 and 2008 were recognised as serotype 6C. In addition, three of eight old isolates originally serotyped as 6A were identified to be serotype 6C. The oldest serotype 6C isolate was from 1962. The serotype 6C isolates belonged to eleven different sequence types (ST) and nine clonal complexes (CC), ST1692 (CC395), ST386 (CC386) and ST481 (CC460) were the predominant types. We tested a novel polyclonal antiserum 6 d, as well as modified antiserum 6b*, provided a scheme for the serotyping of S. pneumoniae serogroup 6 using the Neufeld test and compared the serotyping method with PCR based methods. The two types of methods provided the same results. In future, it will, therefore, be possible to test also serotype 6C in accordance to the standard method for serotyping of S. pneumoniae recommended by WHO. Among all invasive isolates from Denmark 2007 and 2008, serotype 6C constituted 29.6% of the original serotype 6A isolates. The serotype 6C isolates were found to be diverse belonging to a number of different STs and CCs of which most have been observed in other countries previously. Serotype 6C is regarded as an "old" serotype being present among S. pneumoniae isolates in Denmark for at least 48 years. The genetic diversity of serotype 6C isolates and their genetic relationship to other serotypes suggested that serotype 6C strains may have arisen from several different independent recombination events involving different parental strains such as serotypes 6A, 6B, 23F and 4.
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