Identification of miRNAs associated with the mechanical response of hepatic stellate cells by miRNA microarray analysis.
Identification of miRNAs associated with the mechanical response of hepatic stellate cells by miRNA microarray analysis.
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DOI：
10.3892/etm.2018.6384
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发表时间：
2018-09
影响因子：
2.7
通讯作者：
Zhu L
中科院分区：
文献类型：
--
作者：
Yi S;Qin X;Luo X;Zhang Y;Liu Z;Zhu L
已有研究表明，肝星状细胞（HSCs）可用于调节肝脏微循环和门脉高压。张应变对HSC的microRNA（miRNA）谱的影响在很大程度上是未知的。本研究旨在探讨张应变对HSC中miRNA表达的影响。分别用抗结蛋白抗体和抗α-SMA抗体免疫荧光染色测定HSC的纯度和活化。使用微阵列分析对具有和不具有拉伸应变处理的HSC（n=3）进行miRNA谱分析。我们鉴定了6个显著差异表达的miRNA（DEM），包括1个下调的（rno-miR-125 b-2- 3 p）和5个上调的（rno-miR-1224、rho-miR-188- 5 p、rho-miR-211- 3 p、rho-miR-3584- 5 p和rho-miR-466 b-5 p），并通过逆转录-定量聚合酶链反应（RT-qPCR）实验验证。进一步分析的DEM显示，许多重要的生物学过程和信号通路被触发在拉伸应变处理的HSC。这些包括与蛋白质结合、凋亡、增殖以及FoxO和Wnt信号通路相关的信号转导机制。总之，本研究提出了特定的DEM在拉伸应变处理的HSC。我们的研究提供了新的基于miRNA的信息，可能会增强我们对导致门静脉高压症的病理生理过程的理解。
It has been suggested that hepatic stellate cells (HSCs) could be used in the regulation of liver microcirculation and portal hypertension. The effects of tensile strain on the microRNA (miRNA) profile of HSCs are largely unknown. In this study, we aimed to explore the changes of miRNA expression in tensile strain-treated HSCs. The purity and activation of HSCs were determined by immunofluorescence staining with antibody against desmin and a-SMA, respectively. miRNA profile analysis was performed on HSCs with and without tensile strain treatment (n=3) using microarray analysis. We identified 6 significantly differentially expressed miRNAs (DEMs), including 1 downregulated (rno-miR-125b-2-3p) and 5 upregulated (rno-miR-1224, rho-miR-188-5p, rho-miR-211-3p, rho-miR-3584-5p and rho-miR-466b-5p), which were validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) experiments. Further analysis of the DEMs revealed that many important biological processes and signal pathways were triggered in tensile strain-treated HSCs. These include the signal transduction mechanisms associated with protein binding, apoptosis, proliferation, and the FoxO and Wnt signaling pathways. In conclusion, this study presents the specific DEMs in tensile strain-treated HSCs. Our study provide novel miRNA-based information that may enhance our understanding of the pathophysiological processes leading to portal hypertension.
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影响因子：
64.5
作者：
Lewis, BP;Shih, IH;Burge, CB
通讯作者：
Burge, CB
影响因子：
13.5
作者：
Roderburg, Christoph;Urban, Gerd-Willem;Luedde, Tom
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Luedde, Tom
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3.7
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Jansen C;Reiberger T;Huang J;Eischeid H;Schierwagen R;Mandorfer M;Anadol E;Schwabl P;Schwarze-Zander C;Warnecke-Eberz U;Strassburg CP;Rockstroh JK;Peck-Radosavljevic M;Odenthal M;Trebicka J
通讯作者：
Trebicka J
影响因子：
13.5
作者：
Lakner, Ashley M.;Steuerwald, Nury M.;Walling, Tracy L.;Ghosh, Sriparna;Li, Ting;McKillop, Iain H.;Russo, Mark W.;Bonkovsky, Herbert L.;Schrum, Laura W.
通讯作者：
Schrum, Laura W.
影响因子：
4.5
作者：
Di Sario, A;Bendia, E;Benedetti, A
通讯作者：
Benedetti, A