Hypotonic buffer induces meiosis and formation of anucleate cytoplasmic islands in the egg of the two‐spotted cricket Gryllus bimaculatus
Hypotonic buffer induces meiosis and formation of anucleate cytoplasmic islands in the egg of the two‐spotted cricket Gryllus bimaculatus
复制标题
低渗缓冲液诱导双斑蟋蟀卵减数分裂和无核细胞质岛的形成
DOI：
10.1034/j.1600-0854.2004.00679.x
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发表时间：
2003
期刊：
影响因子：
4.6
通讯作者：
S. Noji
中科院分区：
文献类型：
--
作者：
I. Sarashina;Yohei Shinmyo;A. Hirose;K. Miyawaki;T. Mito;H. Ohuchi;T. Horio;S. Noji
在昆虫中，已知卵子激活发生在体内且独立于受精，但其机制尚不清楚。为了进一步了解这些机制，我们尝试在体外激活双斑马鱼卵。经低渗缓冲液处理后，未受精卵减数分裂恢复并完成。研究了活化、未受精卵的早期发育过程，并与受精卵进行了比较。有丝分裂没有进展，导致无核细胞质岛（假能）的形成。激活的未受精卵的发育在这个阶段停止，蛋黄细分和细胞化都没有发生。为了阐明细胞核在受精卵发育到合胞期的作用，用阿霉素处理受精卵以抑制DNA聚合。研究发现，在这些阿霉素处理过的受精卵中也形成了假能。这些结果表明，假能体的数量可以独立于细胞核而增加，这表明在双胞鱼发育到合胞期的过程中起主要作用的是细胞质而不是细胞核。
In insects, egg activation is known to occur in vivo and independently of fertilization, but its mechanisms are poorly understood. To gain understanding of these mechanisms, an attempt was made to activate the egg of Gryllus bimaculatus in vitro. It was found that meiosis resumed and was completed in unfertilized eggs treated with hypotonic buffer. Early developmental processes in activated, unfertilized eggs were investigated and compared with those in fertilized eggs. Mitosis did not progress, resulting in formation of anucleate cytoplasmic islands (pseudoenergids). Development in the activated, unfertilized eggs stopped at this stage and both yolk subdivision and cellularization did not occur. To elucidate the role of the nucleus in the developmental process to the syncytial stage in fertilized eggs, eggs were treated with aphidicolin to inhibit DNA polymerization. It was found that pseudoenergids also formed in these aphidicolin‐treated fertilized eggs. These results demonstrate that pseudoenergids can increase in number independently of nuclei, suggesting that the cytoplasm rather than the nucleus plays the primary role in development to the syncytial stage in G. bimaculatus.
影响因子：
2.7
作者：
Page, AW;OrrWeaver, TL
通讯作者：
OrrWeaver, TL