Role of miR-191/425 cluster in tumorigenesis and diagnosis of gastric cancer.
Role of miR-191/425 cluster in tumorigenesis and diagnosis of gastric cancer.
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DOI：
10.3390/ijms15034031
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发表时间：
2014-03-05
影响因子：
5.6
通讯作者：
Liu ZB
中科院分区：
文献类型：
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作者：
Peng WZ;Ma R;Wang F;Yu J;Liu ZB
胃癌（GC）是世界范围内最常见的癌症类型之一。因此，了解GC肿瘤发生的生物学对于适当的诊断和患者监测是重要的。miR-191/425簇已被报道在多种人类癌症中过表达，但miR-191/425过表达在胃癌发生中的致瘤作用和临床意义目前尚不明确。本研究检测胃癌组织和血清中miR-191和miR-425的表达，并分析其与临床病理资料的关系。我们发现miR-191和miR-425在人胃癌组织中相对于相邻的正常对照均显著增加。此外，miR-191水平与GC肿瘤分期和转移状态相关。此外，当使用血清miR-16作为内源性对照时，GC组中血清miR-191的水平显著高于对照组。最后，在GC细胞系HGC-27中抑制miR-191或miR-425不仅降低细胞增殖和细胞周期进展，而且损害细胞迁移和侵袭。综上所述，我们的研究结果揭示了miR-191和miR-425在胃癌发生中的致癌作用，并表明血清miR-191作为一种新的和稳定的胃癌诊断生物标志物的潜在用途。
Gastric cancer (GC) is among the most frequent types of cancer worldwide. Therefore, understanding the biology of GC tumorigenesis is important for appropriate diagnosis and patient surveillance. The miR-191/425 cluster has been reported to be overexpressed in various human cancers, but the tumorigenic role and clinical significance of miR-191/425 overexpression in gastric carcinogenesis is currently undefined. In this study, the expression of miR-191 and miR-425 in GC tissue and serum was assessed, and the relationship between miRNA expression and clinicopathological data was analyzed. We found that miR-191 and miR-425 were both significantly increased in human GC tissues relative to adjacent normal controls. In addition, miR-191 levels correlated with GC tumor stage and metastatic state. Furthermore, the level of serum miR-191 was significantly higher in the GC group than in the control group when using serum miR-16 as an endogenous control. Finally, inhibition of miR-191 or miR-425 in the GC cell lines HGC-27 not only reduced cell proliferation and cell cycle progression but also impaired cell migration and invasion. Taken together, our results revealed the oncogenic roles of miR-191 and miR-425 in gastric carcinogenesis, and indicated the potential use of serum miR-191 as a novel and stable biomarker for GC diagnosis.
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影响因子：
11.2
作者：
Elyakim, Eran;Sitbon, Einat;Yerushalmi, Noga
通讯作者：
Yerushalmi, Noga
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
4.5
作者：
Di Leva G;Piovan C;Gasparini P;Ngankeu A;Taccioli C;Briskin D;Cheung DG;Bolon B;Anderlucci L;Alder H;Nuovo G;Li M;Iorio MV;Galasso M;Santhanam R;Marcucci G;Perrotti D;Powell KA;Bratasz A;Garofalo M;Nephew KP;Croce CM
通讯作者：
Croce CM
鉴定血清 microRNA 作为胃癌早期检测的新型非侵入性生物标志物。
影响因子：
3.7
作者：
Song MY;Pan KF;Su HJ;Zhang L;Ma JL;Li JY;Yuasa Y;Kang D;Kim YS;You WC
通讯作者：
You WC
影响因子：
8.8
作者：
通讯作者：
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