Circulating microRNAs in plasma of patients with gastric cancers.
Circulating microRNAs in plasma of patients with gastric cancers.
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DOI：
10.1038/sj.bjc.6605608
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发表时间：
2010-03-30
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
我们检测了胃癌（GC）患者的血浆microRNA（miRNA）浓度，以评估其在诊断和监测疾病方面的临床应用。我们首先研究了血浆miRNA检测的适当性，然后将血浆miRNA结果与8名GC患者的癌组织中的表达进行比较，并比较了10名GC患者手术前后配对样本的血浆miRNA。然后，分析了69名GC患者和30名健康志愿者的血浆miRNAs（miR-17- 5 p、miR-21、miR-106 a、miR-106 b和let-7a）。初步分析表明，miRNA在所有血浆样本中均稳定且可检测，并且在大多数情况下，血浆miRNA水平反映了肿瘤miRNA。这些miRNAs的水平在术后样本中显著降低。在大规模分析中，胃癌患者血浆中miR-17- 5 p、miR-21、miR-106 a、miR-106 b的浓度显著高于对照组（分别为P=0.05、0.006、0.008和<0.001），而let-7a在胃癌患者中较低（P=0.002）。miR-106 b测定的受试者工作特征曲线下面积值为0.721，miR-106 a/let-7a比值测定的受试者工作特征曲线下面积值为0.879。循环中miRNAs的检测可能为GC提供新的互补肿瘤标志物。
We examined plasma microRNA (miRNA) concentrations from patients with gastric cancers (GCs) to assess their clinical application for diagnosing and monitoring diseases. We initially investigated the appropriateness of plasma miRNA assay, and then compared plasma miRNA results with the expressions in cancer tissues from eight GC patients, and also compared plasma miRNAs between pre- and post-operative paired samples from 10 GC patients. Then, plasma miRNAs (miR-17-5p, miR-21, miR-106a, miR-106b and let-7a) were analysed in 69 GC patients and 30 healthy volunteers in total. The initial analysis showed that miRNAs were stable and detectable in all plasma samples, and the plasma miRNA levels reflected the tumour miRNAs in most cases. The levels of these miRNAs were significantly reduced in post-operative samples. In large-scale analysis, the plasma concentrations of miRNAs (miR-17-5p, miR-21, miR-106a, miR-106b) were significantly higher in GC patients than controls (P=0.05, 0.006, 0.008 and <0.001 respectively), whereas let-7a was lower in GC patients (P=0.002). The values of the area under the receiver-operating characteristic curve were 0.721 for the miR-106b assay and 0.879 for the miR-106a/let-7a ratio assay. Detection of circulating miRNAs might provide new complementary tumour markers for GC.
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影响因子：
4.7
作者：
Resnick, Kimberly E.;Alder, Hansjuerg;Cohn, David E.
通讯作者：
Cohn, David E.
影响因子：
64.8
作者：
Lu, J;Getz, G;Golub, TR
通讯作者：
Golub, TR
DOI：
10.1158/1078-0432.ccr-08-3131
发表时间：
2009-04-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Avissar M;Christensen BC;Kelsey KT;Marsit CJ
通讯作者：
Marsit CJ
DOI：
10.1111/j.1440-1746.2008.05666.x
发表时间：
2009-04-01
影响因子：
4.1
作者：
Guo, Junming;Miao, Ying;Wang, Yanjun
通讯作者：
Wang, Yanjun
影响因子：
56.9
作者：
Sidransky, D
通讯作者：
Sidransky, D