Investigation of the Material Basis Underlying the Correlation between Presbycusis and Kidney Deficiency in Traditional Chinese Medicine via GC/MS Metabolomics.
Investigation of the Material Basis Underlying the Correlation between Presbycusis and Kidney Deficiency in Traditional Chinese Medicine via GC/MS Metabolomics.
复制标题
通过GC/MS代谢组学研究中药老年性耳聋与肾虚相关的物质基础。
DOI：
10.1155/2013/762092
复制
发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Shi JR
中科院分区：
文献类型：
--
作者：
Dong Y;Ding Y;Liu PZ;Song HY;Zhao YP;Li M;Shi JR
客观的。从代谢角度探讨老年性耳聋与中医肾虚的相关性及其物质基础。方法。纯音测听用于测试听觉功能。采用肾虚症状评分表来衡量研究对象的肾虚累积评分。采用气相色谱/质谱法（GC/MS）测定了11名老年性耳聋患者和9名听力正常老年人的尿液样本中的代谢物。结果。老年人听力损失与中医肾虚评分呈正相关。老年性耳聋患者和对照组之间的尿液代谢物谱存在显着差异。共发现23种差异表达代谢物。京都基因与基因组百科全书（KEGG）通路分析显示，这些代谢物与谷胱甘肽代谢、氨基酸代谢、葡萄糖代谢、N-甲基-D-天冬氨酸（NMDA）受体通路、γ-氨基丁酸（GABA）受体通路有关。结论。谷胱甘肽代谢、氨基酸代谢、葡萄糖代谢、NMDA受体、GABA受体可能与老年性耳聋的发病机制有关，可能是老年性耳聋与中医肾虚相关的物质基础。
Objective. To investigate the correlation between presbycusis and kidney deficiency as defined by traditional Chinese medicine (TCM) and its material basis from the perspective of metabolism. Methods. Pure-tone audiometry was used to test auditory function. A kidney deficiency symptom scoring table was used to measure the kidney deficiency accumulated scores of the research subjects. Gas chromatography/mass spectrometry (GC/MS) was used to measure the metabolites in the urine samples from 11 presbycusis patients and 9 elderly people with normal hearing. Results. Hearing loss in the elderly was positively correlated with kidney deficiency score in TCM. There were significant differences in urine metabolite profile between the presbycusis patients and the controls. A total of 23 differentially expressed metabolites were found. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed that these metabolites were related to glutathione metabolism, amino acid metabolism, glucose metabolism, the N-methyl-D-aspartic acid (NMDA) receptor pathway, and the γ-aminobutyric acid (GABA) receptor pathway. Conclusion. Glutathione metabolism, amino acid metabolism, glucose metabolism, NMDA receptors, and GABA receptors may be related to the pathogenesis of presbycusis and may be the material basis underlying the correlation between presbycusis and kidney deficiency in TCM.
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DOI：
10.1155/2012/968083
发表时间：
2012
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Wu T;Yang M;Wei HF;He SH;Wang SC;Ji G
通讯作者：
Ji G
DOI：
10.1155/2013/509134
发表时间：
2013
期刊：
Evidence-based complementary and alternative medicine : eCAM
影响因子：
--
作者：
Cui HZ;Wang LM;Zhao X;Liu YY;Wang SX;Li XH;Jiang YM;Chen JX
通讯作者：
Chen JX
影响因子：
3.3
作者：
Caspary, DM;Holder, TM;Naritoku, DK
通讯作者：
Naritoku, DK
影响因子：
4.2
作者：
Tanaka C;Coling DE;Manohar S;Chen GD;Hu BH;Salvi R;Henderson D
通讯作者：
Henderson D
影响因子：
3.7
作者：
Bradshaw-Pierce EL;Pitts TM;Kulikowski G;Selby H;Merz AL;Gustafson DL;Serkova NJ;Eckhardt SG;Weekes CD
通讯作者：
Weekes CD