Gnp4/LAX2, a RAWUL protein, interferes with the OsIAA3-OsARF25 interaction to regulate grain length via the auxin signaling pathway in rice.
Gnp4/LAX2, a RAWUL protein, interferes with the OsIAA3-OsARF25 interaction to regulate grain length via the auxin signaling pathway in rice.
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DOI：
10.1093/jxb/ery256
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发表时间：
2018-09-14
影响因子：
6.9
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Zhang Z;Li J;Tang Z;Sun X;Zhang H;Yu J;Yao G;Li G;Guo H;Li J;Wu H;Huang H;Xu Y;Yin Z;Qi Y;Huang R;Yang W;Li Z
Gnp4/LAX2干扰OsIAA3-OsARF25的相互作用，激活OsERF142/SMOS1的表达，从而调节水稻的粒长。粒长是水稻产量的决定因素之一，生长素通过调节细胞生长对粒长起着重要的调节作用。虽然生长素途径中的几个基因参与调节籽粒长度，但其潜在的分子机制仍不清楚。在这项研究中，我们鉴定了一个环指和WD40相关的泛素样域(RAWUL)蛋白Gnp4/LAX2，它通过影响细胞扩张来调节谷粒长度，到目前为止还不清楚。Gnp4/LAX2在植物中广泛表达，亚细胞定位分析表明它编码一种核蛋白。过量表达Gnp4/LAX2能显著增加籽粒长度和千粒重。此外，Gnp4/LAX2与OsIAA3在物理上相互作用，从而在体内外干扰OsIAA3-OsARF25的相互作用。OsIAA3 RNAi植物始终表现出较长的颗粒，而突变体osarf25的颗粒较小。此外，OsARF25还与器官大小调节因子OsERF142/SMOS1的启动子结合，并正向调节其表达。综上所述，Gnp4/LAX2通过参与OsIAA3-OsARF25-OsERF142途径调控籽粒长度，在水稻分子育种中具有潜在的应用价值。
Gnp4/LAX2 interferes with the OsIAA3–OsARF25 interaction to activate the expression of OsERF142/SMOS1, and hence regulates grain length in rice. Grain length is one of the determinants of yield in rice and auxin plays an important role in regulating it by mediating cell growth. Although several genes in the auxin pathway are involved in regulating grain length, the underlying molecular mechanisms remain unclear. In this study we identify a RING-finger and wd40-associated ubiquitin-like (RAWUL) domain-containing protein, Gnp4/LAX2, with a hitherto unknown role in regulation of grain length by its influence on cell expansion. Gnp4/LAX2 is broadly expressed in the plant and subcellular localization analysis shows that it encodes a nuclear protein. Overexpression of Gnp4/LAX2 can significantly increase grain length and thousand-kernel weight. Moreover, Gnp4/LAX2 physically interacts with OsIAA3 and consequently interferes with the OsIAA3–OsARF25 interaction in vitro and in vivo. OsIAA3 RNAi plants consistently exhibit longer grains, while the mutant osarf25 has small grains. In addition, OsARF25 binds to the promoter of OsERF142/SMOS1, a regulator of organ size, and positively regulates its expression. Taken together, the results reveal that Gnp4/LAX2 functions as a regulator of grain length through participation in the OsIAA3–OsARF25–OsERF142 pathway and that it has potential value for molecular breeding in rice.
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