Differential sRNA regulation in leaves and roots of sugarcane under water depletion.
Differential sRNA regulation in leaves and roots of sugarcane under water depletion.
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DOI：
10.1371/journal.pone.0093822
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Ferreira PC
中科院分区：
文献类型：
--
作者：
Thiebaut F;Grativol C;Tanurdzic M;Carnavale-Bottino M;Vieira T;Motta MR;Rojas C;Vincentini R;Chabregas SM;Hemerly AS;Martienssen RA;Ferreira PC
植物已经发展了多种调节机制来响应和适应胁迫。干旱胁迫是全球农业生产力的主要限制因素之一，最近的报告强调了植物sRNA在响应和适应水资源供应方面的重要性。为了增加我们对sRNA在响应水分耗竭中的作用的理解，对甘蔗品种进行停止滴灌24小时的处理。对不同干旱敏感性甘蔗品种的叶片和根系进行了sRNA深度测序分析。选择的sRNA库允许分析不同的sRNA类别（miRNA和siRNA）。在叶和根文库中分别鉴定出28个和36个保守的miRNA家族。通过茎环qRT-PCR分析，观察了miRNA的动态调节，并在来自三个生物学重复的叶片样品中验证了8种miRNA的表达谱，所述叶片样品使用品种：SP 90 -1638敏感品种; SP 83 -2847和SP 83 -5073耐受品种。改变的miRNA调控与特定靶点的mRNA水平变化相关。两个叶片库，从个别甘蔗品种对比耐旱性能进行了分析。在叶片文库中观察到22-nt sRNA种类的富集。22-nt miRNA通过响应于水消耗而切割其靶标来触发siRNA产生。sRNA生物合成途径的一些基因在耐受基因型中下调，而在敏感基因型中上调，以响应水分耗竭处理。我们的分析有助于增加对sRNA在甘蔗缺水中的作用的认识。
Plants have developed multiple regulatory mechanisms to respond and adapt to stress. Drought stress is one of the major constraints to agricultural productivity worldwide and recent reports have highlighted the importance of plant sRNA in the response and adaptation to water availability. In order to increase our understanding of the roles of sRNA in response to water depletion, cultivars of sugarcane were submitted to treatment of ceasing drip irrigation for 24 hours. Deep sequencing analysis was carried out to identify the sRNA regulated in leaves and roots of sugarcane cultivars with different drought sensitivities. The pool of sRNA selected allowed the analysis of different sRNA classes (miRNA and siRNA). Twenty-eight and 36 families of conserved miRNA were identified in leaf and root libraries, respectively. Dynamic regulation of miRNA was observed and the expression profiles of eight miRNA were verified in leaf samples from three biological replicates by stem-loop qRT-PCR assay using the cultivars: SP90–1638 - sensitive cultivar- and; SP83–2847 and SP83–5073 - tolerant cultivars. Altered miRNA regulation was correlated with changes in mRNA levels of specific targets. Two leaf libraries from individual sugarcane cultivars with contrasting drought-tolerance properties were also analyzed. An enrichment of 22-nt sRNA species was observed in leaf libraries. 22-nt miRNA triggered siRNA production by cleavage of their targets in response to water depletion. A number of genes of the sRNA biogenesis pathway were down-regulated in tolerant genotypes and up-regulated in sensitive in response to water depletion treatment. Our analysis contributes to increase the knowledge on the roles of sRNA in sugarcane submitted to water depletion.
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影响因子：
3.7
作者：
Ferreira TH;Gentile A;Vilela RD;Costa GG;Dias LI;Endres L;Menossi M
通讯作者：
Menossi M
影响因子：
14.9
作者：
Chen C;Ridzon DA;Broomer AJ;Zhou Z;Lee DH;Nguyen JT;Barbisin M;Xu NL;Mahuvakar VR;Andersen MR;Lao KQ;Livak KJ;Guegler KJ
通讯作者：
Guegler KJ
影响因子：
9.5
作者：
Guleria, Praveen;Mahajan, Monika;Bhardwaj, Jyoti;Yadav, Sudesh Kumar
通讯作者：
Yadav, Sudesh Kumar
影响因子：
4.4
作者：
Cantu D;Vanzetti LS;Sumner A;Dubcovsky M;Matvienko M;Distelfeld A;Michelmore RW;Dubcovsky J
通讯作者：
Dubcovsky J
影响因子：
3.7
作者：
Carnavale Bottino M;Rosario S;Grativol C;Thiebaut F;Rojas CA;Farrineli L;Hemerly AS;Ferreira PC
通讯作者：
Ferreira PC