microRNAs associated with drought response in the bioenergy crop sugarcane (Saccharum spp.).
microRNAs associated with drought response in the bioenergy crop sugarcane (Saccharum spp.).
复制标题
DOI：
10.1371/journal.pone.0046703
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Menossi M
中科院分区：
文献类型：
--
作者：
Ferreira TH;Gentile A;Vilela RD;Costa GG;Dias LI;Endres L;Menossi M
甘蔗（Sugarcane）是世界上最重要的农作物之一。干旱胁迫是影响甘蔗产量的主要非生物胁迫因子。然而，介导植物对水分胁迫的反应的基因网络在许多作物物种中仍然是未知的。尽管在其他物种中已经描述了在水分胁迫期间介导转录后调节的几种microRNA，但甘蔗microRNA在干旱胁迫期间的作用尚未研究。本研究的目的是鉴定在干旱胁迫下差异表达的甘蔗miRNAs，并将这种表达与两个耐旱性不同的甘蔗品种的行为相关联。甘蔗品种RB 867515（耐旱性较高）和RB 855536（耐旱性较低）在温室中栽培三个月，然后经受干旱2、4、6或8天。通过对小RNA的深度测序，我们能够鉴定出18个miRNA家族。在所有这样鉴定的miRNAs中，有7个在干旱期间差异表达。其中6个miRNAs在应激2天后差异表达，5个miRNAs在应激4天后差异表达。通过RT-qPCR（定量逆转录酶PCR）验证了五种miRNA（ssp-miR 164、ssp-miR 394、ssp-miR 397、ssp-miR 399-seq 1和miR 528）的表达水平。使用计算机模拟方法预测了差异表达的miRNA的6个前体和靶标，并通过RT-qPCR进行了验证;其中许多靶标可能在耐旱性中发挥重要作用。这些发现构成了甘蔗中鉴定的miRNAs数量的显著增加，并有助于阐明干旱胁迫激活的复杂调控网络。
Sugarcane (Saccharum spp.) is one of the most important crops in the world. Drought stress is a major abiotic stress factor that significantly reduces sugarcane yields. However the gene network that mediates plant responses to water stress remains largely unknown in several crop species. Although several microRNAs that mediate post-transcriptional regulation during water stress have been described in other species, the role of the sugarcane microRNAs during drought stress has not been studied. The objective of this work was to identify sugarcane miRNAs that are differentially expressed under drought stress and to correlate this expression with the behavior of two sugarcane cultivars with different drought tolerances. The sugarcane cultivars RB867515 (higher drought tolerance) and RB855536 (lower drought tolerance) were cultivated in a greenhouse for three months and then subjected to drought for 2, 4, 6 or 8 days. By deep sequencing of small RNAs, we were able to identify 18 miRNA families. Among all of the miRNAs thus identified, seven were differentially expressed during drought. Six of these miRNAs were differentially expressed at two days of stress, and five miRNAs were differentially expressed at four days. The expression levels of five miRNAs (ssp-miR164, ssp-miR394, ssp-miR397, ssp-miR399-seq 1 and miR528) were validated by RT-qPCR (quantitative reverse transcriptase PCR). Six precursors and the targets of the differentially expressed miRNA were predicted using an in silico approach and validated by RT-qPCR; many of these targets may play important roles in drought tolerance. These findings constitute a significant increase in the number of identified miRNAs in sugarcane and contribute to the elucidation of the complex regulatory network that is activated by drought stress.
登录
查看更多内容
影响因子：
14.9
作者：
Dai X;Zhao PX
通讯作者：
Zhao PX
影响因子：
4.2
作者：
Dillon SL;Shapter FM;Henry RJ;Cordeiro G;Izquierdo L;Lee LS
通讯作者：
Lee LS
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
5.1
作者：
Collinge, M;Boller, T
通讯作者：
Boller, T
DOI：
10.1073/pnas.191389398
发表时间：
2001-09-25
影响因子：
11.1
作者：
Gaxiola, RA;Li, JS;Fink, GR
通讯作者：
Fink, GR