RNase I Modulates Escherichia coli Motility, Metabolism, and Resistance.
RNase I Modulates Escherichia coli Motility, Metabolism, and Resistance.
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DOI：
10.1021/acschembio.0c00390
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发表时间：
2020-07-17
影响因子：
4
通讯作者：
Weinert EE
中科院分区：
文献类型：
--
作者：
Duggal Y;Fontaine BM;Dailey DM;Ning G;Weinert EE
细菌通过感知细胞外因素来不断适应环境，这些细胞外因素触发了细胞内信号分子的产生，被称为第二信使。最近在大肠杆菌中发现了2‘3，’-环核苷酸单磷酸盐(2‘，3’-cNMPs)，并可能成为新的信号分子。2‘，3’-cNMPs是由T2核糖核酸酶RNaseI内切短RNA产生的，但其生理功能尚不清楚。我们的转录分析表明，RNaseI参与调节许多细胞过程，包括核苷酸代谢、运动性、酸敏感性、金属动态平衡和外膜形态。通过缺失菌株和抑制剂的组合研究，我们证明了RNaseI在大肠杆菌应激抗性中发挥着以前未知的作用，它通过影响细菌在受到包括抗生素在内的外部威胁时所采用的防御机制的一部分。因此，这项工作提供了对RNaseI在细菌信号和生理中的新角色的洞察，并突出了RNaseI作为抗菌佐剂靶点的潜力。
Bacteria are constantly adapting to their environment by sensing extracellular factors that trigger production of intracellular signaling molecules, known as second messengers. Recently, 2’3,’-cyclic nucleotide monophosphates (2’,3’-cNMPs) were identified in Escherichia coli and have emerged as possible novel signaling molecules. 2’,3’-cNMPs are produced through endonucleolytic cleavage of short RNAs by the T2 endoribonuclease, RNase I; however, the physiological roles of RNase I remain unclear. Our transcriptomic analysis suggests that RNase I is involved in regulating numerous cellular processes, including nucleotide metabolism, motility, acid sensitivity, metal homeostasis and outer membrane morphology. Through a combination of deletion strain and inhibitor studies, we demonstrate that RNase I plays a previously unknown role in E. coli stress resistance by affecting pathways that are part of the defense mechanisms employed by bacteria when introduced to external threats, including antibiotics. Thus, this work provides insight into the emerging roles of RNase I in bacterial signaling and physiology and highlights the potential of RNase I as a target for antibacterial adjuvants.
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