Iron-responsive chromatin remodelling and MAPK signalling enhance adhesion in Candida albicans.
Iron-responsive chromatin remodelling and MAPK signalling enhance adhesion in Candida albicans.
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DOI：
10.1111/mmi.12659
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发表时间：
2014-07
影响因子：
3.6
通讯作者：
Edgerton M
中科院分区：
文献类型：
--
作者：
Puri S;Lai WK;Rizzo JM;Buck MJ;Edgerton M
最近的累积数据表明，在致病真菌白色念珠菌中，各种转录因子以铁响应的方式被招募到染色质中，以影响不同的细胞功能。在这里，我们通过基因启动子上的染色质重塑分析，使用微球菌核酸酶(MNase)消化和深度测序，鉴定了白色念珠菌中的铁反应基因群。在铁充足条件下，铁吸收利用基因启动子区域的染色质分别呈现抑制和活性构型。对各自启动子区域染色质差异重塑基因的GO术语富集性分析表明，许多与黏附有关的基因也是铁反应的。观察到，在铁存在的情况下，白色念珠菌具有更强的自我粘附性(增加2倍)，并形成更高的生物被膜质量(增加77%)。此外，我们鉴定了多种已知的和新的黏附相关基因，这些基因具有铁依赖的活性染色质图谱，表明在铁充足的条件下潜在的上调。被Cek1磷酸化激活的转录因子Cph1在铁充足的条件下也表现出活跃的染色质图谱，细胞在铁存在的情况下表现出铁响应的Cek1 MAPK磷酸化。因此，在白色念珠菌中，铁通过调节大基因的染色质分布和通过Cek1 MAPK信号转导而影响多种生物学功能。
Recent cumulative data shows that various transcription factors are recruited to the chromatin in an iron responsive manner to affect diverse cellular functions in the pathogenic fungus Candida albicans. Here we identified groups of iron-responsive genes in C. albicans by chromatin remodeling analysis at gene promoters, using micrococcal nuclease (MNase) digestion followed by deep sequencing. Chromatin in the promoter regions of iron uptake and utilization genes showed repressed and active configuration, respectively, under iron replete conditions. GO Term enrichment analysis of genes with differentially remodeled chromatin, in respective promoter locales, suggested that many genes involved in adhesion are also iron-responsive. C. albicans was observed to be more self-adherent (2-fold increase) and formed higher biofilm mass (77% increase) in the presence of iron. Furthermore, we identified various known and novel adhesion-related genes with iron-dependent active chromatin profiles that are indicative of potential up-regulation under iron replete conditions. Transcription factor Cph1 that is activated upon Cek1 phosphorylation also showed an active chromatin profile under iron replete conditions and cells showed iron-responsive Cek1 MAPK phosphorylation in the presence of iron. Thus, iron affects diverse biological functions by modulating chromatin profiles of large gene sets and by signaling through Cek1 MAPK in C. albicans.
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影响因子：
30.3
作者：
Chen C;Pande K;French SD;Tuch BB;Noble SM
通讯作者：
Noble SM
影响因子：
9.8
作者：
Jung WH;Sham A;White R;Kronstad JW
通讯作者：
Kronstad JW
影响因子：
30.8
作者：
Buck, Michael J.;Lieb, Jason D.
通讯作者：
Lieb, Jason D.
影响因子：
3.4
作者：
Garhammer, P;Hiller, K-A;Schmalz, G
通讯作者：
Schmalz, G
影响因子：
--
作者：
Hsu, Po-Chen;Chao, Chun-Cheih;Lan, Chung-Yu
通讯作者：
Lan, Chung-Yu