Hydrolysis of amelogenin by matrix metalloprotease-20 accelerates mineralization in vitro.
Hydrolysis of amelogenin by matrix metalloprotease-20 accelerates mineralization in vitro.
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DOI：
10.1016/j.archoralbio.2011.06.016
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发表时间：
2011-12
影响因子：
3
通讯作者：
Habelitz, Stefan
中科院分区：
文献类型：
--
作者：
Uskokovic, Vuk;Khan, Feroz;Liu, Haichuan;Witkowska, Halina Ewa;Zhu, Li;Li, Wu;Habelitz, Stefan
在以下方面，牙釉质是哺乳动物体内独特的组织：（a）它是其中矿化最多和最硬的组织，包含高达95重量%的磷灰石;（B）它的微结构主要由平行杆组成，所述平行杆由40 - 60 nm宽的磷灰石晶体束组成，纵横比高达1：10，000和（c）产生釉质的蛋白质基质不仅引导晶体生长，而且还平行地进行其自身的降解和去除。因此，当在体外模拟釉质形成过程时，晶体生长必须与已知在进行适当晶体生长中起关键作用的釉质蛋白组装体的蛋白水解消化相结合。实验设置的基础上控制和可编程滴定的釉原蛋白溶胶消化的MMP-20与缓冲的钙和磷酸盐溶液被用来模拟细长的，板状晶体的形成。虽然釉原蛋白可以单独作为成核和晶体生长的促进剂，在这项研究中，我们表明，蛋白水解发挥了额外的成核和生长促进作用。MMP-20对全长釉原蛋白的水解减少了蛋白质和肽粘附并覆盖基底所需的临界时间。蛋白质层的形成和固定随后减少了磷酸钙结晶的时间。在0.4 mg/ml rH 174存在下，将蛋白水解反应与滴定相结合，已显示对晶体生长的促进作用与将rH 174的浓度增加四倍至1.6 mg/ml相同。因此，控制蛋白水解裂解的速率和程度可用于控制蛋白质引导的结晶系统中的成核和生长速率。
In the following respects, tooth enamel is a unique tissue in the mammalian body: (a) it is the most mineralized and hardest tissue in it comprising up to 95 wt% of apatite; (b) its microstructure is dominated by parallel rods composed of bundles of 40 – 60 nm wide apatite crystals with aspect ratios reaching up to 1:10,000 and (c) not only does the protein matrix that gives rise to enamel guides the crystal growth, but it also conducts its own degradation and removal in parallel. Hence, when mimicking the process of amelogenesis in vitro, crystal growth has to be coupled to proteolytic digestion of the amelogenin assemblies that are known to play a pivotal role in conducting the proper crystal growth. Experimental settings based on controlled and programmable titration of amelogenin sols digested by means of MMP-20 with buffered calcium and phosphate solutions were employed to imitate the formation of elongated, plate-shaped crystals. While amelogenin can act as a promoter of nucleation and crystal growth alone, in this study we show that proteolysis exerts an additional nucleation- and growth-promoting effect. Hydrolysis of full-length amelogenin by MMP-20 decreases the critical time needed for the protein and peptides to adhere and to cover the substrate. The formation and immobilization of a protein layer subsequently reduces the time for calcium phosphate crystallization. Coupling the proteolytic reaction to titration in the presence of 0.4 mg/ml rH174 has been shown to have the same effect on the crystal growth promotion as quadrupling the concentration of rH174 to 1.6 mg/ml. Controlling the rate and the extent of the proteolytic cleavage can thus be used to control the nucleation and growth rates in a protein-guided crystallization system.
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影响因子：
3
作者：
He, Xiaodong;Li, Wu;Habelitz, Stefan
通讯作者：
Habelitz, Stefan
影响因子：
4.2
作者：
Lacruz, Rodrigo S.;Nanci, Antonio;Kurtz, Ira;Wright, J. Timothy;Paine, Michael L.
通讯作者：
Paine, Michael L.
影响因子：
4.2
作者：
AOBA, T;FUKAE, M;MORENO, EC
通讯作者：
MORENO, EC
影响因子：
2.9
作者：
Snead, ML
通讯作者：
Snead, ML
影响因子：
2.9
作者：
Delak, Katya;Harcup, Craig;Lakshminarayanan, Rajamani;Sun, Zhi;Fan, Yuwwei;Moradian-Oldak, Janet;Evans, John Spencer
通讯作者：
Evans, John Spencer