Microparticle content of plasma for transfusion is influenced by the whole blood hold conditions: pre-analytical considerations for proteomic investigations.
Microparticle content of plasma for transfusion is influenced by the whole blood hold conditions: pre-analytical considerations for proteomic investigations.
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DOI：
10.1016/j.jprot.2012.07.013
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发表时间：
2012-12-05
影响因子：
3.3
通讯作者：
Chan KS
中科院分区：
文献类型：
--
作者：
Sparrow RL;Chan KS
微粒（MPs）从正常血细胞中脱落，可能有助于血浆的凝血潜能。输血新鲜冷冻血浆（FFP）用于纠正创伤或大手术中的凝血功能障碍和失血。MPs在FFP临床疗效中的作用尚不清楚。不同国家对FFP编制的管理条例各不相同。本研究的目的是确定FFP制备前全血（WB）保持条件对MP谱的影响。在制备FFP之前，WB单元在室温（RT）或RT和冷藏的组合下保存长达24小时。测定解冻后FFP中MP的含量，以反映输血实践。随着WB保持时间的延长，FFP中MPs的绝对数量增加。WB的制冷也可能促进MPs的生成。特别是血小板衍生的和含有磷脂酰丝氨酸的MPs（已知具有促凝特性）的数量增加。脂质过氧化随着wb保持时间的延长而增加。供体相关因素似乎控制脂质过氧化水平。FFP MPs的整体蛋白质组学和凝固分析是必要的。这些信息可以指导选择WB的最佳处理条件和FFP最相关的质量控制程序。
Microparticles (MPs) are shed from normal blood cells and may contribute to the coagulation potential of plasma. Transfusion of fresh frozen plasma (FFP) is used to correct coagulopathies and blood loss in trauma or major surgery. The role of MPs in FFP clinical efficacy is unknown. Regulations that govern the preparation of FFP vary in different countries. The aim of this study was to determine the effect of whole blood (WB)-hold conditions before FFP preparation on the MP profile. WB units were held at room temperature (RT) or combination of RT and refrigeration for up to 24hr before FFP preparation. The MP content in thawed FFP was measured to reflect transfusion practice. The absolute number of MPs in FFP increased with longer WB hold time. Refrigeration of WB may also promote increased generation of MPs. In particular the number of platelet-derived and phosphatidylserine-containing MPs, which are known to have procoagulant properties, increased. Lipid peroxidation increased with longer WB-hold time. Donor-related factors appear to govern lipid peroxidation levels. Holistic proteomic and coagulant analyses of FFP MPs is warranted. Such information could guide the choice of the optimal handling conditions of WB and the most relevant quality control procedures for FFP.
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影响因子：
4.4
作者：
Tuck MK;Chan DW;Chia D;Godwin AK;Grizzle WE;Krueger KE;Rom W;Sanda M;Sorbara L;Stass S;Wang W;Brenner DE
通讯作者：
Brenner DE
影响因子：
2
作者：
Greening, David W.;Glenister, Kristen M.;Simpson, Richard J.
通讯作者：
Simpson, Richard J.
影响因子：
5.7
作者：
Rak, Janusz
通讯作者：
Rak, Janusz
DOI：
10.1016/j.transci.2009.10.008
发表时间：
2010-02
期刊：
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis
影响因子：
--
作者：
Rumjantseva V;Hoffmeister KM
通讯作者：
Hoffmeister KM
DOI：
10.1097/ta.0b013e318232e5ab
发表时间：
2011-11-01
影响因子：
--
作者：
Rizoli, Sandro B.;Scarpelini, Sandro;Tien, Homer C.
通讯作者：
Tien, Homer C.