Buffalo (Bubalus bubalis) interleukin-2: sequence analysis reveals high nucleotide and amino acid identity with interleukin-2 of cattle and other ruminants.
Buffalo (Bubalus bubalis) interleukin-2: sequence analysis reveals high nucleotide and amino acid identity with interleukin-2 of cattle and other ruminants.
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水牛（Bubalus bubalis）白细胞介素-2：序列分析显示与牛和其他反刍动物的白细胞介素-2 具有高度的核苷酸和氨基酸一致性。
DOI：
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
T. Rasool
中科院分区：
文献类型：
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作者：
E. Sreekumar;A. Premraj;M. Saravanakumar;T. Rasool
采用聚合酶链反应（PCR）技术扩增并克隆了印度布法罗白细胞介素-2（IL-2）基因的4400 bp基因组序列和332 bp截短的cDNA序列。从基因组克隆和截短的cDNA克隆的5'端组装布法罗IL-2基因的编码序列。该序列与牛IL-2的核苷酸同源性为98.5%，氨基酸同源性为98%。在位置63、124和135处观察到三个氨基酸取代。预测的布法罗IL-2蛋白质结构与人和牛IL-2蛋白质结构的比较没有发现显著差异。负责IL-2受体结合的推定氨基酸在布法罗、牛和人IL-2中是保守的。布法罗IL-2的氨基酸序列与其它反刍动物IL-2的氨基酸序列也具有很高的同源性，表明其具有功能交叉反应性。
A 4400-bp genomic sequence and a 332-bp truncated cDNA sequence of the interleukin-2 (IL-2) gene of Indian water buffalo (Bubalus bubalis) were amplified by polymerase chain reaction and cloned. The coding sequence of the buffalo IL-2 gene was assembled from the 5' end of the genomic clone and the truncated cDNA clone. This sequence had 98.5% nucleotide identity and 98% amino acid identity with cattle IL-2. Three amino acid substitutions were observed at positions 63, 124 and 135. Comparison of the predicted protein structure of buffalo IL-2 with that of human and cattle IL-2 did not reveal significant differences. The putative amino acids responsible for IL-2 receptor binding were conserved in buffalo, cattle and human IL-2. The amino acid sequence of buffalo IL-2 also showed very high identity with that of other ruminants, indicating functional cross-reactivity.
鉴定白细胞介素 2 和 p64 白细胞介素 2 受体 γ 链之间的直接相互作用。
DOI：
10.1073/pnas.90.6.2428
发表时间：
1993
影响因子：
11.1
作者：
Voss,SD;Leary,TP;Sondel,PM;Robb,RJ
通讯作者：
Robb,RJ
功能性牛白细胞介素 2 cDNA 的分子克隆。
DOI：
10.1073/pnas.83.10.3228
发表时间：
1986
影响因子：
11.1
作者：
Reeves,R;Spies,AG;Nissen,MS;Buck,CD;Weinberg,AD;Barr,PJ;Magnuson,NS;Magnuson,JA
通讯作者：
Magnuson,JA