Label-free, real-time detection of perineural invasion and cancer margins in a murine model of head and neck cancer surgery.
Label-free, real-time detection of perineural invasion and cancer margins in a murine model of head and neck cancer surgery.
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DOI：
10.1038/s41598-022-16975-w
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发表时间：
2022-07-27
影响因子：
4.6
通讯作者：
St John, Maie
中科院分区：
文献类型：
--
作者：
Tam, Kenric;Alhiyari, Yazeed;Huang, Shan;Han, Albert;Stafsudd, Oscar;Shori, Ramesh;St John, Maie
头颈部肿瘤的外科治疗需要在恶性肿瘤的完全切除和功能保留之间保持谨慎的平衡。外科医生还必须确定是否切除重要的脑神经，因为牺牲神经可能导致严重的发病率，包括面瘫。我们的小组以前曾报道，动态光学对比成像（DOCI），一种新的非侵入性成像系统，可以确定恶性和健康组织之间的边缘。在此，我们使用体内小鼠模型来证明DOCI可以准确地识别癌症边缘和神经周围浸润，与伴随组织学一致。将8只C3 H/HeJ雄性小鼠的双侧胁腹皮下注射SCCVIISF（一种鼠头颈癌细胞系）。在切除前进行DOCI成像以确定切缘。将肿瘤和边缘都送去进行组织切片。在确认DOCI可以描述HNSCC边缘后，我们研究了DOCI是否可以识别PNI。在6只C3 H/HeJ雄性小鼠中，左侧坐骨神经注射PBS，右侧注射SCCVISF。DOCI成像后，收集坐骨神经进行组织学分析。所有DOCI图像均在术中实时采集（每个通道10 s），具有手术相关的宽视野。区分癌与邻近健康组织类型的DOCI值具有统计学显著性（P < 0.05）。DOCI成像也能够以100%的准确性检测神经周围侵犯，与对照组相比（P < 0.05）。DOCI允许术中实时可视化恶性和健康组织边缘以及神经周围浸润，以帮助指导肿瘤切除。
Surgical management of head and neck cancer requires a careful balance between complete resection of malignancy and preservation of function. Surgeons must also determine whether to resect important cranial nerves that harbor perineural invasion (PNI), as sacrificing nerves can result in significant morbidity including facial paralysis. Our group has previously reported that Dynamic Optical Contrast Imaging (DOCI), a novel non-invasive imaging system, can determine margins between malignant and healthy tissues. Herein, we use an in vivo murine model to demonstrate that DOCI can accurately identify cancer margins and perineural invasion, concordant with companion histology. Eight C3H/HeJ male mice were injected subcutaneously into the bilateral flanks with SCCVIISF, a murine head and neck cancer cell line. DOCI imaging was performed prior to resection to determine margins. Both tumor and margins were sent for histologic sectioning. After validating that DOCI can delineate HNSCC margins, we investigated whether DOCI can identify PNI. In six C3H/HeJ male mice, the left sciatic nerve was injected with PBS and the right with SCCVIISF. After DOCI imaging, the sciatic nerves were harvested for histologic analysis. All DOCI images were acquired intraoperatively and in real-time (10 s per channel), with an operatively relevant wide field of view. DOCI values distinguishing cancer from adjacent healthy tissue types were statistically significant (P < 0.05). DOCI imaging was also able to detect perineural invasion with 100% accuracy compared to control (P < 0.05). DOCI allows for intraoperative, real-time visualization of malignant and healthy tissue margins and perineural invasion to help guide tumor resection.
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DOI：
10.1073/pnas.0708425104
发表时间：
2007-12-04
影响因子：
11.1
作者：
Skala, Melissa C.;Riching, Kristin M.;Ramanujam, Nirmala
通讯作者：
Ramanujam, Nirmala
影响因子：
1.1
作者：
Melo, Giulianno Molina de;Cervantes, Onivaldo;Abrahao, Marcio
通讯作者：
Abrahao, Marcio
影响因子：
3.7
作者：
Shah, Amy T.;Beckler, Michelle Demory;Skala, Melissa C.
通讯作者：
Skala, Melissa C.
影响因子：
3.2
作者：
Pan, Jiongru;Deng, Han;Wang, Yuxin
通讯作者：
Wang, Yuxin
影响因子：
254.7
作者：
Siegel, Rebecca L.;Miller, Kimberly D.;Jemal, Ahmedin
通讯作者：
Jemal, Ahmedin